Functional characterization of Hsp33 protein from Bacillus psychrosaccharolyticus; additional function of HSP33 on resistance to solvent stress

Hyun Jun Kang, Dong Hyuk Heo, So Woong Choi, Kyung Nam Kim, Jaekyung Shim, Chan Wha Kim, Ha Chin Sung, Cheol-Won Yun

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Psychrophiles have been known as efficient organism to degrade organic solvent. To investigate the mechanism of solvent stress and identify the factors that affect the solvent stress in psychrophiles, we selected Bacillus psychrosaccharolyticus one of the psychrophiles and two-dimensional gel electrophoresis was performed. Among the protein spots analyzed by 2-DE, five spots induced in 3% IPA stress conditions were identified by MS/MS, and one of these spots was identified as a Hsp33 family. The Hsp33 protein sequence of B. psychrosaccharolyticus exhibited a high similarity with the corresponding proteins of other bacteria. The Hsp33 protein of B. psychrosaccharolyticus has a highly conserved zinc-binding domain (CXCX, CXXC) that includes four cysteine residues in the C-terminus. In addition, the transcriptional induction of the HSP33 of B. psychrosaccharolyticus was confirmed by Northern blot analysis, and formation of free thiol linkage was induced under stress conditions such as exposure to solvents, heat-shock, and oxidative stress. Furthermore, over-expressed strains of HSP33 of B. psychrosaccharolyticus in Escherichia coli improved stress tolerance to the organic solvent when compared with the wild-type. These data suggest that the solvent stress condition was similar to heat-shock or oxidative stress, especially through the triggering of induction and activation of a redox-regulatory chaperone, Hsp33, and Hsp33 plays a critical role in the tolerance to stress.

Original languageEnglish
Pages (from-to)743-750
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume358
Issue number3
DOIs
Publication statusPublished - 2007 Jul 6

Fingerprint

Bacilli
Bacillus
Proteins
Oxidative stress
Shock
Oxidative Stress
Organic solvents
Hot Temperature
Electrophoresis, Gel, Two-Dimensional
Sulfhydryl Compounds
Northern Blotting
Oxidation-Reduction
Cysteine
Zinc
Electrophoresis
Escherichia coli
Bacteria
Gels
Chemical activation

Keywords

  • Bacillus psychrosaccharolyticus
  • Hsp33
  • Psychrophiles
  • Solvent tolerance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Functional characterization of Hsp33 protein from Bacillus psychrosaccharolyticus; additional function of HSP33 on resistance to solvent stress. / Kang, Hyun Jun; Heo, Dong Hyuk; Choi, So Woong; Kim, Kyung Nam; Shim, Jaekyung; Kim, Chan Wha; Sung, Ha Chin; Yun, Cheol-Won.

In: Biochemical and Biophysical Research Communications, Vol. 358, No. 3, 06.07.2007, p. 743-750.

Research output: Contribution to journalArticle

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