Functional characterization of pectin methylesterase inhibitor (PMEI) in wheat

Min Jeong Hong, Dae Yeon Kim, Tong Geon Lee, Woong Bae Jeon, Yong Weon Seo

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Pectin, one of the main components of plant cell wall, is deesterified by the pec- tin methylesterase (PME). PME activity is regulated by inhibitor proteins known as the pectin methylesterase inhibitor (PMEI), which plays a key role in wound- ing, osmotic stress, senescence and seed development. However, the role of PMEI in many plant species still remains to be elucidated, especially in wheat. To facil- itate the expression analysis of the TaPMEI gene, RT-PCR was performed using leaf, stem and root tissues that were treated with exogeneous application of phy- tohormones and abiotic stresses. High transcription was detected in salicylic acid (SA) and hydrogen peroxide treatments. To elucidate the subcellular localization of the TaPMEI protein, the TaPMEI:GFP fusion construct was transformed into onion epidermal cells by particle bombardment. The fluorescence signal was exclusively detected in the cell wall. Using an enzyme assay, we confirmed that PME was completely inhibited by TaPMEI. These results indicated that TaPMEI was involved in inhibition of pectin methylesterification and may play a role in the plant defense mechanism via cell wall fortification.

Original languageEnglish
Pages (from-to)97-106
Number of pages10
JournalGenes and Genetic Systems
Volume85
Issue number2
DOIs
Publication statusPublished - 2010

Keywords

  • 2BS/2RL translocation line
  • Cell wall
  • Pectin methylesterase (pme)
  • Pectin methylesterase inhibitor (pmei)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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