Functional characterization of resting and adenovirus-induced reactive astrocytes in three-dimensional culture

Junsung Woo, Sun Kyoung Im, Heejung Chun, Soon Young Jung, Soo Jin Oh, Nakwon Choi, Changjoon Lee, Eun Mi Hur

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Brain is a rich environment where neurons and glia interact with neighboring cells as well as extracellular matrix in three-dimensional (3D) space. Astrocytes, which are the most abundant cells in the mammalian brain, reside in 3D space and extend highly branched processes that form microdomains and contact synapses. It has been suggested that astrocytes cultured in 3D might be maintained in a less reactive state as compared to those growing in a traditional, two-dimensional (2D) monolayer culture. However, the functional characterization of the astrocytes in 3D culture has been lacking. Here we cocultured neurons and astrocytes in 3D and examined the morphological, molecular biological, and electrophysiological properties of the 3D-cultured hippocampal astrocytes. In our 3D neuron-astrocyte coculture, astrocytes showed a typical morphology of a small soma with many branches and exhibited a unique membrane property of passive conductance, more closely resembling their native in vivo counterparts. Moreover, we also induced reactive astrocytosis in culture by infecting with high-titer adenovirus to mimic pathophysiological conditions in vivo . Adenoviral infection induced morphological changes in astrocytes, increased passive conductance, and increased GABA content as well as tonic GABA release, which are characteristics of reactive gliosis. Together, our study presents a powerful in vitro model resembling both physiological and pathophysiological conditions in vivo , and thereby provides a versatile experimental tool for studying various neurological diseases that accompany reactive astrocytes.

Original languageEnglish
Pages (from-to)158-167
Number of pages10
JournalExperimental Neurobiology
Volume26
Issue number3
DOIs
Publication statusPublished - 2017 Jun 1
Externally publishedYes

Fingerprint

Adenoviridae
Astrocytes
Gliosis
Neurons
gamma-Aminobutyric Acid
Brain
Carisoprodol
Coculture Techniques
Neuroglia
Synapses
Extracellular Matrix
Membranes
Infection

Keywords

  • 3D culture
  • Adenovirus
  • Functional characterization
  • Passive conductance
  • Reactive astrocyte
  • Tonic GABA current

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Functional characterization of resting and adenovirus-induced reactive astrocytes in three-dimensional culture. / Woo, Junsung; Im, Sun Kyoung; Chun, Heejung; Jung, Soon Young; Oh, Soo Jin; Choi, Nakwon; Lee, Changjoon; Hur, Eun Mi.

In: Experimental Neurobiology, Vol. 26, No. 3, 01.06.2017, p. 158-167.

Research output: Contribution to journalArticle

Woo, Junsung ; Im, Sun Kyoung ; Chun, Heejung ; Jung, Soon Young ; Oh, Soo Jin ; Choi, Nakwon ; Lee, Changjoon ; Hur, Eun Mi. / Functional characterization of resting and adenovirus-induced reactive astrocytes in three-dimensional culture. In: Experimental Neurobiology. 2017 ; Vol. 26, No. 3. pp. 158-167.
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