Functional conservation and diversification between rice OsMADS22/OsMADS55 and Arabidopsis SVP proteins

Jeong Hwan Lee, Soo Hyun Park, Ji Hoon Ahn

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

MADS-box transcription factors play pivotal roles in several aspects of plant growth and development. The Arabidopsis SHORT VEGETATIVE PHASE (SVP) protein mediates the integration of signals involved in the control of flowering time and flower development by interacting with MADS-box proteins. In the rice genome, three SVP-like genes (OsMADS22, OsMADS47, and OsMADS55) are present. To investigate the functional conservation of these SVP-like genes in rice and Arabidopsis, the phenotypes of transgenic Arabidopsis plants overexpressing OsMADS22 and OsMADS55 were analyzed. Overexpression of OsMADS22 and OsMADS55 led to abnormal floral morphologies including leaf-like sepals, whereas only OsMADS55 expression caused delayed flowering via downregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). Yeast two-hybrid assays revealed that OsMADS22 and OsMADS55 interacted with Arabidopsis AGL24 and AP1, but only OsMADS55 interacted with FLC. Overexpression of OsMADS55, but not OsMADS22, complemented the early flowering phenotype and ambient temperature-insensitive flowering phenotype seen in svp mutants, suggesting that OsMADS55 regulates flowering time associated with ambient temperature responses in Arabidopsis. Taken together, our data are consistent with functional conservation and diversification between Arabidopsis and rice SVP-like genes involved in controlling flowering time and flower development.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalPlant Science
Volume185-186
DOIs
Publication statusPublished - 2012 Apr 1

Fingerprint

Arabidopsis
flowering
rice
Proteins
proteins
Phenotype
phenotype
Genes
ambient temperature
Two-Hybrid System Techniques
Temperature
Plant Development
Genetically Modified Plants
Growth and Development
two hybrid system techniques
genes
Oryza
Transcription Factors
Down-Regulation
calyx

Keywords

  • Flower development
  • Flowering time
  • OsMADS22
  • OsMADS55
  • Rice
  • SVP

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Agronomy and Crop Science

Cite this

Functional conservation and diversification between rice OsMADS22/OsMADS55 and Arabidopsis SVP proteins. / Lee, Jeong Hwan; Park, Soo Hyun; Ahn, Ji Hoon.

In: Plant Science, Vol. 185-186, 01.04.2012, p. 97-104.

Research output: Contribution to journalArticle

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