Functional expression of AMPA receptors on central terminals of rat dorsal root ganglion neurons and presynaptic inhibition of glutamate release

C. Justin Lee, Rita Bardoni, Chi Kun Tong, Holly Sue Engelman, Donald J. Joseph, Pier Cosimo Magherini, Amy B. MacDermott

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Abstract

No direct evidence has been found for expression of functional AMPA receptors by dorsal root ganglion neurons despite immunocytochemical evidence suggesting they are present. Here we report evidence for expression of functional AMPA receptors by a subpopulation of dorsal root ganglion neurons. The AMPA receptors are most prominently located near central terminals of primary afferent fibers. AMPA and kainate receptors were detected by recording receptor-mediated depolarization of the central terminals under selective pharmacological conditions. We demonstrate that activation of presynaptic AMPA receptors by exogenous agonists causes inhibition of glutamate release from the terminals, possibly via primary afferent depolarization (PAD). These results challenge the traditional view that GABA and GABAA receptors exclusively mediate PAD, and indicate that PAD is also mediated by glutamate acting on presynaptically localized AMPA and kainate receptors.

Original languageEnglish
Pages (from-to)135-146
Number of pages12
JournalNeuron
Volume35
Issue number1
DOIs
Publication statusPublished - 2002 Jul 3

ASJC Scopus subject areas

  • Neuroscience(all)

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    Lee, C. J., Bardoni, R., Tong, C. K., Engelman, H. S., Joseph, D. J., Magherini, P. C., & MacDermott, A. B. (2002). Functional expression of AMPA receptors on central terminals of rat dorsal root ganglion neurons and presynaptic inhibition of glutamate release. Neuron, 35(1), 135-146. https://doi.org/10.1016/S0896-6273(02)00729-8