Functional expression of single-chain variable fragment antibody against c-Met in the cytoplasm of Escherichia coli

Mi Ae Heo, Su Hyun Kim, So Yeon Kim, Yu Jin Kim, Junho Chung, Min-Kyu Oh, Sun Gu Lee

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

c-Met, a high affinity receptor for hepatocyte growth factor/scatter factor, shown to be overexpressed in a variety of malignant cells, is a potential biomarker as well as a therapeutic target. Thus, single-chain antibody fragment (scFv) specific for c-Met is expected to be efficiently employed in the clinical treatment or imaging of many cancer cells. Here, we constructed the expression system for anti-c-Met scFv fused with T7 tag at its N-terminus using pET vector and investigated the expression conditions to achieve a functional and soluble expression of the scFv in the cytoplasm of recombinant Escherichia coli. The redox potential of E. coli cytoplasm was the most critical factor for the functional expression of anti-c-Met scFv. The employment of a host with oxidizing cytoplasm, E. coli trxB/gor double mutant, improved the productivity of functional anti-c-Met scFv by approximately 10-fold compared to the production of anti-c-Met scFv in the reducing cytoplasm of wild type E. coli. Productivity of functional anti-c-Met scFv could be further enhanced by co-expressing molecular chaperones such as GroELS, trigger factor, and DsbC with the scFv. Coexpression of DsbC increased the yield of functional anti-c-Met scFv about 2.5-fold in the cytoplasm of E. coli trxB/gor mutant compared to the production of scFv without DsbC coexpression. Lowering the IPTG concentration from 1 to 0.05 mM led to the slight enhancement, approximately 1.6-fold, of productivity of functional scFv. Although the use of low temperature for anti-c-Met scFv expression increased the ratio of soluble scFv fraction to insoluble fraction, productivity of soluble scFv decreased owing to the significant reduction of expression rate. The addition of 0.5 M sucrose in the medium inhibited the formation of intracellular insoluble anti-c-Met scFv. To purify the anti-c-Met scFv simply, we fused hexahistidine at the C-terminus of scFv and purified the scFv showing 98% of purity through the interaction between Ni2+ and histidine.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalProtein Expression and Purification
Volume47
Issue number1
DOIs
Publication statusPublished - 2006 May 1

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Single-Chain Antibodies
Escherichia coli
Cytoplasm
Productivity
His-His-His-His-His-His
Proto-Oncogene Proteins c-met
Isopropyl Thiogalactoside
Immunoglobulin Fragments
Molecular Chaperones
Hepatocyte Growth Factor
Biomarkers
Histidine
Oxidation-Reduction
Sucrose
Cells
Imaging techniques
Temperature
Neoplasms

Keywords

  • Anti-c-Met scFv
  • Folding
  • Molecular chaperones
  • Soluble expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Functional expression of single-chain variable fragment antibody against c-Met in the cytoplasm of Escherichia coli. / Heo, Mi Ae; Kim, Su Hyun; Kim, So Yeon; Kim, Yu Jin; Chung, Junho; Oh, Min-Kyu; Lee, Sun Gu.

In: Protein Expression and Purification, Vol. 47, No. 1, 01.05.2006, p. 203-209.

Research output: Contribution to journalArticle

Heo, Mi Ae ; Kim, Su Hyun ; Kim, So Yeon ; Kim, Yu Jin ; Chung, Junho ; Oh, Min-Kyu ; Lee, Sun Gu. / Functional expression of single-chain variable fragment antibody against c-Met in the cytoplasm of Escherichia coli. In: Protein Expression and Purification. 2006 ; Vol. 47, No. 1. pp. 203-209.
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