Functional integration of serial dilution and capillary electrophoresis on a PDMS microchip

Jun Keun Chang, Yun Seok Heo, Hyunwoo Bang, Keunchang Cho, Seok Chung, Chanil Chung, Dong Chul Han

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

For the quantitative analysis of an unknown sample a calibration curve should be obtained, as analytical instruments give relative, rather than absolute measurements. Therefore, researchers should make standard samples with various known concentrations, measure each standard and the unknown sample, and then determine the concentration of the unknown by comparing the measured value to those of the standards. These procedures are tedious and time-consuming. Therefore, we developed a polymer based microfluidic device from polydimethylsiloxane, which integrates serial dilution and capillary electrophoresis functions in a single device. The integrated microchip can provide a one-step analytical tool, and thus replace the complex experimental procedures. Two plastic syringes, one containing a buffer solution and the other a standard solution, were connected to two inlet holes on a microchip, and pushed by a hydrodynamic force. The standard sample is serially diluted to various concentrations through the microfluidic networks. The diluted samples are sequentially introduced through microcha n-nels by electro-osmotic force, and their laser-induced fluorescence signals measured by capillary electrophoresis. We demonstrate the integrated microchip performance by measuring the fluorescence signals of fluorescein at various concentrations. The calibration curve obtained from the electropherograms showed the expected linearity.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume8
Issue number4
Publication statusPublished - 2003 Dec 1
Externally publishedYes

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Dilution
Lab-On-A-Chip Devices
Microfluidics
Calibration
Fluorescence
Syringes
Polydimethylsiloxane
Hydrodynamics
Fluorescein
Plastics
Buffers
Polymers
Lasers
Research Personnel
Equipment and Supplies
Chemical analysis

Keywords

  • Capillary electrophoresis
  • Lab-on-a-chip
  • Microchip
  • Polydimethylsiloxane (PDMS)
  • Serial dilution

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology

Cite this

Chang, J. K., Heo, Y. S., Bang, H., Cho, K., Chung, S., Chung, C., & Han, D. C. (2003). Functional integration of serial dilution and capillary electrophoresis on a PDMS microchip. Biotechnology and Bioprocess Engineering, 8(4), 233-239.

Functional integration of serial dilution and capillary electrophoresis on a PDMS microchip. / Chang, Jun Keun; Heo, Yun Seok; Bang, Hyunwoo; Cho, Keunchang; Chung, Seok; Chung, Chanil; Han, Dong Chul.

In: Biotechnology and Bioprocess Engineering, Vol. 8, No. 4, 01.12.2003, p. 233-239.

Research output: Contribution to journalArticle

Chang, JK, Heo, YS, Bang, H, Cho, K, Chung, S, Chung, C & Han, DC 2003, 'Functional integration of serial dilution and capillary electrophoresis on a PDMS microchip', Biotechnology and Bioprocess Engineering, vol. 8, no. 4, pp. 233-239.
Chang, Jun Keun ; Heo, Yun Seok ; Bang, Hyunwoo ; Cho, Keunchang ; Chung, Seok ; Chung, Chanil ; Han, Dong Chul. / Functional integration of serial dilution and capillary electrophoresis on a PDMS microchip. In: Biotechnology and Bioprocess Engineering. 2003 ; Vol. 8, No. 4. pp. 233-239.
@article{f97d9523ec0d4b7696398154657c417f,
title = "Functional integration of serial dilution and capillary electrophoresis on a PDMS microchip",
abstract = "For the quantitative analysis of an unknown sample a calibration curve should be obtained, as analytical instruments give relative, rather than absolute measurements. Therefore, researchers should make standard samples with various known concentrations, measure each standard and the unknown sample, and then determine the concentration of the unknown by comparing the measured value to those of the standards. These procedures are tedious and time-consuming. Therefore, we developed a polymer based microfluidic device from polydimethylsiloxane, which integrates serial dilution and capillary electrophoresis functions in a single device. The integrated microchip can provide a one-step analytical tool, and thus replace the complex experimental procedures. Two plastic syringes, one containing a buffer solution and the other a standard solution, were connected to two inlet holes on a microchip, and pushed by a hydrodynamic force. The standard sample is serially diluted to various concentrations through the microfluidic networks. The diluted samples are sequentially introduced through microcha n-nels by electro-osmotic force, and their laser-induced fluorescence signals measured by capillary electrophoresis. We demonstrate the integrated microchip performance by measuring the fluorescence signals of fluorescein at various concentrations. The calibration curve obtained from the electropherograms showed the expected linearity.",
keywords = "Capillary electrophoresis, Lab-on-a-chip, Microchip, Polydimethylsiloxane (PDMS), Serial dilution",
author = "Chang, {Jun Keun} and Heo, {Yun Seok} and Hyunwoo Bang and Keunchang Cho and Seok Chung and Chanil Chung and Han, {Dong Chul}",
year = "2003",
month = "12",
day = "1",
language = "English",
volume = "8",
pages = "233--239",
journal = "Biotechnology and Bioprocess Engineering",
issn = "1226-8372",
publisher = "Korean Society for Biotechnology and Bioengineering",
number = "4",

}

TY - JOUR

T1 - Functional integration of serial dilution and capillary electrophoresis on a PDMS microchip

AU - Chang, Jun Keun

AU - Heo, Yun Seok

AU - Bang, Hyunwoo

AU - Cho, Keunchang

AU - Chung, Seok

AU - Chung, Chanil

AU - Han, Dong Chul

PY - 2003/12/1

Y1 - 2003/12/1

N2 - For the quantitative analysis of an unknown sample a calibration curve should be obtained, as analytical instruments give relative, rather than absolute measurements. Therefore, researchers should make standard samples with various known concentrations, measure each standard and the unknown sample, and then determine the concentration of the unknown by comparing the measured value to those of the standards. These procedures are tedious and time-consuming. Therefore, we developed a polymer based microfluidic device from polydimethylsiloxane, which integrates serial dilution and capillary electrophoresis functions in a single device. The integrated microchip can provide a one-step analytical tool, and thus replace the complex experimental procedures. Two plastic syringes, one containing a buffer solution and the other a standard solution, were connected to two inlet holes on a microchip, and pushed by a hydrodynamic force. The standard sample is serially diluted to various concentrations through the microfluidic networks. The diluted samples are sequentially introduced through microcha n-nels by electro-osmotic force, and their laser-induced fluorescence signals measured by capillary electrophoresis. We demonstrate the integrated microchip performance by measuring the fluorescence signals of fluorescein at various concentrations. The calibration curve obtained from the electropherograms showed the expected linearity.

AB - For the quantitative analysis of an unknown sample a calibration curve should be obtained, as analytical instruments give relative, rather than absolute measurements. Therefore, researchers should make standard samples with various known concentrations, measure each standard and the unknown sample, and then determine the concentration of the unknown by comparing the measured value to those of the standards. These procedures are tedious and time-consuming. Therefore, we developed a polymer based microfluidic device from polydimethylsiloxane, which integrates serial dilution and capillary electrophoresis functions in a single device. The integrated microchip can provide a one-step analytical tool, and thus replace the complex experimental procedures. Two plastic syringes, one containing a buffer solution and the other a standard solution, were connected to two inlet holes on a microchip, and pushed by a hydrodynamic force. The standard sample is serially diluted to various concentrations through the microfluidic networks. The diluted samples are sequentially introduced through microcha n-nels by electro-osmotic force, and their laser-induced fluorescence signals measured by capillary electrophoresis. We demonstrate the integrated microchip performance by measuring the fluorescence signals of fluorescein at various concentrations. The calibration curve obtained from the electropherograms showed the expected linearity.

KW - Capillary electrophoresis

KW - Lab-on-a-chip

KW - Microchip

KW - Polydimethylsiloxane (PDMS)

KW - Serial dilution

UR - http://www.scopus.com/inward/record.url?scp=33644984512&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644984512&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 233

EP - 239

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

SN - 1226-8372

IS - 4

ER -