Abstract
Two chondrogenic factors, Dex and TGF-β1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-β1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage-specific genes and clearly displayed type-II collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF-β1.
| Original language | English |
|---|---|
| Pages (from-to) | 221-229 |
| Number of pages | 9 |
| Journal | Macromolecular Bioscience |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2009 Mar 10 |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomaterials
- Materials Chemistry
- Polymers and Plastics
Cite this
Functional PLGA scaffolds for chondrogenesis of bone-marrow-derived mesenchymal stem cells. / Park, Kwideok; Cho, Kyoung J.; Kim, Jae J.; Kim, Ik Hwan; Han, Dong Keun.
In: Macromolecular Bioscience, Vol. 9, No. 3, 10.03.2009, p. 221-229.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional PLGA scaffolds for chondrogenesis of bone-marrow-derived mesenchymal stem cells
AU - Park, Kwideok
AU - Cho, Kyoung J.
AU - Kim, Jae J.
AU - Kim, Ik Hwan
AU - Han, Dong Keun
PY - 2009/3/10
Y1 - 2009/3/10
N2 - Two chondrogenic factors, Dex and TGF-β1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-β1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage-specific genes and clearly displayed type-II collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF-β1.
AB - Two chondrogenic factors, Dex and TGF-β1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-β1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage-specific genes and clearly displayed type-II collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF-β1.
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UR - http://www.scopus.com/inward/citedby.url?scp=65549134284&partnerID=8YFLogxK
U2 - 10.1002/mabi.200800187
DO - 10.1002/mabi.200800187
M3 - Article
C2 - 19089870
AN - SCOPUS:65549134284
VL - 9
SP - 221
EP - 229
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
SN - 1616-5187
IS - 3
ER -