Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing

Ki Jeong Kim, Jee Hyuck Lim, Sanghyeob Lee, Young Jin Kim, Soo Bok Choi, Min Kyung Lee, Doil Choi, Kyung Hee Paek

Research output: Contribution to journalArticle

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Abstract

A series of microarray analyses employing the expressed sequence tags (ESTs) of hot pepper was conducted in an effort to elucidate the molecular mechanisms inherent to hypersensitive response (HR) by viral or bacterial pathogens. There were 2535 ESTs exhibiting differential expression (over 2-fold changes) among about 5000 ESTs during viral or bacterial response. Further, via virus-induced gene silencing (VIGS) and TMV-infection studies, we were able to isolate several ESTs, which may be relevant to defense response against TMV. Of these ESTs, Capsicum annuum fatty acid desaturase 1 (CaFAD1) showed the distinct phenotype against TMV infection and thus was subjected to further study. CaFAD1-silenced plants showed weaker resistance against TMV-P0 infection compared to TRV2 control plants. Also the suppression of FAD1 expression caused blocking of cell death induced by Bcl2-associated X (Bax) protein in tobacco plants. Therefore, this report presents that both microarray and VIGS approaches are feasible in hot pepper plants and the TMV-induced CaFAD1 plays a role in HR response.

Original languageEnglish
Pages (from-to)554-561
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume362
Issue number3
DOIs
Publication statusPublished - 2007 Oct 26

Fingerprint

Fatty Acid Desaturases
Tobacco Mosaic Virus
Capsicum
Tobacco
Expressed Sequence Tags
Gene Silencing
Microarrays
Viruses
Complementary DNA
Clone Cells
Genes
Infection
bcl-2-Associated X Protein
Pathogens
Cell death
Microarray Analysis
Cell Death
Phenotype

Keywords

  • Capsicum annuum
  • Fatty acid desaturase
  • TMV
  • Virus-induced gene silencing

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing. / Kim, Ki Jeong; Lim, Jee Hyuck; Lee, Sanghyeob; Kim, Young Jin; Choi, Soo Bok; Lee, Min Kyung; Choi, Doil; Paek, Kyung Hee.

In: Biochemical and Biophysical Research Communications, Vol. 362, No. 3, 26.10.2007, p. 554-561.

Research output: Contribution to journalArticle

Kim, Ki Jeong ; Lim, Jee Hyuck ; Lee, Sanghyeob ; Kim, Young Jin ; Choi, Soo Bok ; Lee, Min Kyung ; Choi, Doil ; Paek, Kyung Hee. / Functional study of Capsicum annuum fatty acid desaturase 1 cDNA clone induced by Tobacco mosaic virus via microarray and virus-induced gene silencing. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 362, No. 3. pp. 554-561.
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