Functional study of hot pepper 26S proteasome subunit RPN7 induced by Tobacco mosaic virus from nuclear proteome analysis

Boo Ja Lee, Sun Jae Kwon, Sung Kyu Kim, Ki Jeong Kim, Chang Jin Park, Young Jin Kim, Ohkmae Kim, Kyung Hee Paek

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Two-dimensional gel electrophoresis (2-DE) was applied for the screening of Tobacco mosaic virus (TMV)-induced hot pepper (Capsicum annuum cv. Bugang) nuclear proteins. From differentially expressed protein spots, we acquired the matched peptide mass fingerprint (PMF) data, analyzed by MALDI-TOF MS, from the non-redundant hot pepper EST protein FASTA database using the VEMS 2.0 software. Among six identified nuclear proteins, the hot pepper 26S proteasome subunit RPN7 (CaRPN7) was subjected to further study. The level of CaRPN7 mRNA was specifically increased during incompatible TMV-P0 interaction, but not during compatible TMV-P1.2 interaction. When CaRPN7::GFP fusion protein was targeted in onion cells, the nuclei had been broken into pieces. In the hot pepper leaves, cell death was exacerbated and genomic DNA laddering was induced by Agrobacterium-mediated transient overexpression of CaPRN7. Thus, this report presents that the TMV-induced CaRPN7 may be involved in programmed cell death (PCD) in the hot pepper plant.

Original languageEnglish
Pages (from-to)405-411
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume351
Issue number2
DOIs
Publication statusPublished - 2006 Dec 15

Fingerprint

Tobacco Mosaic Virus
Capsicum
Tobacco
Proteome
Viruses
Cell death
Nuclear Proteins
Proteins
Cell Death
Electrophoresis
Protein Databases
Screening
Agrobacterium
Peptide Mapping
Onions
Fusion reactions
Gels
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Cell Nucleus

Keywords

  • CaRPN7
  • EST database
  • Hot pepper
  • Hypersensitive response
  • Nuclear protein
  • Programmed cell death
  • Proteasome
  • Proteomics
  • Subcellular localization
  • TMV

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Functional study of hot pepper 26S proteasome subunit RPN7 induced by Tobacco mosaic virus from nuclear proteome analysis. / Lee, Boo Ja; Kwon, Sun Jae; Kim, Sung Kyu; Kim, Ki Jeong; Park, Chang Jin; Kim, Young Jin; Kim, Ohkmae; Paek, Kyung Hee.

In: Biochemical and Biophysical Research Communications, Vol. 351, No. 2, 15.12.2006, p. 405-411.

Research output: Contribution to journalArticle

Lee, Boo Ja ; Kwon, Sun Jae ; Kim, Sung Kyu ; Kim, Ki Jeong ; Park, Chang Jin ; Kim, Young Jin ; Kim, Ohkmae ; Paek, Kyung Hee. / Functional study of hot pepper 26S proteasome subunit RPN7 induced by Tobacco mosaic virus from nuclear proteome analysis. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 351, No. 2. pp. 405-411.
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