Fusion protein of retinol-binding protein and albumin domain III reduces liver fibrosis

Hongsik Lee, Hyeyeun Jeong, Sangeun Park, Wonbaek Yoo, Soyoung Choi, Kyungmin Choi, Min Goo Lee, Mihwa Lee, Daeryong Cha, Young Sik Kim, Jeeyoung Han, Wonkon Kim, Sun Hwa Park, Junseo Oh

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Activated hepatic stellate cells (HSCs) play a key role in liver fibrosis, and inactivating HSCs has been considered a promising therapeutic approach. We previously showed that albumin and its derivative designed for stellate cell-targeting, retinol-binding protein-albumin domain III fusion protein (referred to as R-III), inactivate cultured HSCs. Here, we investigated the mechanism of action of albumin/R-III in HSCs and examined the anti-fibrotic potential of R-III in vivo. R-III treatment and albumin expression downregulated retinoic acid (RA) signaling which was involved in HSC activation. RA receptor agonist and retinaldehyde dehydrogenase overexpression abolished the anti-fibrotic effect of R-III and albumin, respectively. R-III uptake into cultured HSCs was significantly decreased by siRNA-STRA6, and injected R-III was localized predominantly in HSCs in liver. Importantly, R-III administration reduced CCl4- and bile duct ligation-induced liver fibrosis. R-III also exhibited a preventive effect against CCl4-inducd liver fibrosis. These findings suggest that the anti-fibrotic effect of albumin/R-III is, at least in part, mediated by downregulation of RA signaling and that R-III is a good candidate as a novel anti-fibrotic drug.

Original languageEnglish
Pages (from-to)819-830
Number of pages12
JournalEMBO Molecular Medicine
Volume7
Issue number6
DOIs
Publication statusPublished - 2015 Jun 1

Keywords

  • Albumin
  • Anti-fibrotic drug
  • Fibrosis
  • Hepatic stellate cell
  • Retinoic acid

ASJC Scopus subject areas

  • Molecular Medicine

Fingerprint Dive into the research topics of 'Fusion protein of retinol-binding protein and albumin domain III reduces liver fibrosis'. Together they form a unique fingerprint.

Cite this