GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47)

Heon Lee Jeong, Bae Rho Seung, Taehoon Chun

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

GABAA receptor-associated protein (GABARAP) is a 14-kDa cytoplasmic protein initially identified as a molecular chaperone for GABA A receptor in cortical neurons. However, evidence indicates that the function of GABARAP is much broader than a specific role in neuronal cells. As an initial step to define the biological role of GABARAP, we searched for binding partners of GABARAP using co-immunoprecipitation coupled with LC-MS/MS analysis. As a result, DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47), recently identified as RNA helicase, is identified as a binding partner of GABARAP. An interaction between GABARAP and DDX 47 was further confirmed by yeast two-hybrid systems. Further, co-transfection of GABARAP and DDX47 cDNA into a tumor cell line induces apoptosis. This result may be the first evidence showing that GABARAP and DDX 47 are involved in the apoptotic process.

Original languageEnglish
Pages (from-to)623-628
Number of pages6
JournalBiotechnology Letters
Volume27
Issue number9
DOIs
Publication statusPublished - 2005 May 1
Externally publishedYes

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Keywords

  • Apoptosis
  • DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47)
  • GABA receptor-associated protein (GABARAP)
  • RNA helicase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

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