GAC1, a gene encoding a putative GTPase-activating protein, regulates bikaverin biosynthesis in Fusarium verticillioides

Yoon-E Choi, Jillian A. Brown, Courtney B. Williams, Lorena L. Canales, Won Bo Shim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Fusarium verticillioides (teleomorph Gibber-ella moniliformis) is an ascomycete known to produce a variety of secondary metabolites, including fumoni-sins, fusaric acid and bikaverin. These metabolites are synthesized when the fungus is under stress, notably nutrient limitations. To date we have limited understanding of the complex regulatory process associated with fungal secondary metabolism. In this study we generated a collection of F. verticillioides mutants by using REMI (restriction enzyme mediated integration) mutagenesis and in the process identified a strain, R647, that carries a mutation in a gene designated GAC1. Mutation in the GAC1 locus, which encodes a putative GTPase activating protein, resulted in the increased production of bikaverin, suggesting that GAC1 is negatively associated with bikaverin biosynthesis. Complementation of R647 with the wild-type GAC1 gene restored the bikaverin production level to that of the wild-type progenitor, demonstrating that gac1 mutation was directly responsible for the overproduction of bikaverin. We also demonstrated that AREA, encoding global nitrogen regulator, and PKS4, encoding polyketide synthase, are downstream genes that respectively are regulated positively and negatively by GAC1. Our results suggest that GAC1 plays an important role in signal transduction regulating bikaverin production in F. verticillioides.

Original languageEnglish
Pages (from-to)701-709
Number of pages9
JournalMycologia
Volume100
Issue number5
DOIs
Publication statusPublished - 2008 Sep 1
Externally publishedYes

Fingerprint

GTPase-activating proteins
GTPase-Activating Proteins
Fusarium
mutation
biosynthesis
protein
gene
fusaric acid
Genes
polyketide synthases
genes
nutrient limitation
secondary metabolite
mutagenesis
Ascomycota
Mutation
secondary metabolites
signal transduction
metabolite
Fusaric Acid

Keywords

  • Restriction enzyme mediated integration
  • Secondary metabolism
  • Signal transduction

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Molecular Biology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

GAC1, a gene encoding a putative GTPase-activating protein, regulates bikaverin biosynthesis in Fusarium verticillioides. / Choi, Yoon-E; Brown, Jillian A.; Williams, Courtney B.; Canales, Lorena L.; Shim, Won Bo.

In: Mycologia, Vol. 100, No. 5, 01.09.2008, p. 701-709.

Research output: Contribution to journalArticle

Choi, Yoon-E ; Brown, Jillian A. ; Williams, Courtney B. ; Canales, Lorena L. ; Shim, Won Bo. / GAC1, a gene encoding a putative GTPase-activating protein, regulates bikaverin biosynthesis in Fusarium verticillioides. In: Mycologia. 2008 ; Vol. 100, No. 5. pp. 701-709.
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