Gamma-irradiation and doxorubicin treatment of normal human cells cause cell cycle arrest via different pathways

Seong Min Lee, BuHyun Youn, Cha Soon Kim, Chong Soon Kim, ChulHee Kang, Joon Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ionizing radiation and doxorubicin both produce oxidative damage and double-strand breaks in DNA. Double-strand breaks and oxidative damage are highly toxic and cause cell cycle arrest, provoking DNA repair and apoptosis in cancer cell lines. To investigate the response of normal human cells to agents causing oxidative damage, we monitored alterations in gene expression in F65 normal human fibroblasts. Treatment with γ-irradiation and doxorubicin altered the expression of 23 and 68 known genes, respectively, with no genes in common. Both agents altered the expression of genes involved in cell cycle arrest, and arrested the treated cells in G2/M phase 12 h after treatment. 24 h after γ-irradiation, the percentage of G1 cells increased, whereas after doxorubicin treatment the percentage of G 2/M cells remained constant for 24 h. Our results suggest that F65 cells respond differently to γ-irradiation- and doxorubicin-induced DNA damage, probably using entirely different biochemical pathways.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalMolecules and Cells
Volume20
Issue number3
Publication statusPublished - 2005 Dec 1

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Keywords

  • γ-Irradiation
  • Differential Gene Expression
  • Doxorubicin
  • Normal Human Fibroblasts

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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