Abstract
The production of chimeric birds is an important tool for the investigation of vertebrate development, the conservation of endangered birds, and the development of various biotechnological applications. This study examined whether gamma (γ)-irradiation depletes endogenous primordial germ cells and enhances the efficiency of somatic chimerism in chickens. An optimal irradiation protocol for stage X embryos was determined after irradiation at various doses (0, 100, 300, 500, 600, 700, and 2,000 rad). Exposure to 500 rad of γ-irradiation for 73 s significantly decreased the number of primordial germ cells (P∈<∈0.0001). Somatic chimera hatchlings were then produced by transferring blastodermal cells from a Korean Oge into either an irradiated (at 500 rad) or intact stage X White Leghorn embryo. An analysis of feather color pattern and polymerase chain reaction-based species-specific amplification of various tissues of the hatchlings confirmed chimerism in most organs of the chick produced from the irradiated recipient; a lesser degree of chimerism was observed in the non-irradiated control recipient. In conclusion, the exposure of chick embryos to an optimized dose of γ-irradiation effectively depleted germ cells and yielded greater somatic chimerism than non-irradiated control embryos. This technique can be applied to interspecies reproduction or the production of transgenic birds.
| Original language | English |
|---|---|
| Pages (from-to) | 828-833 |
| Number of pages | 6 |
| Journal | In Vitro Cellular and Developmental Biology - Animal |
| Volume | 46 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2010 Dec 1 |
| Externally published | Yes |
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Keywords
- Chimera
- Gamma (γ)-irradiation
- Primordial germ cells (PGCs)
- Stage X embryos
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Gamma-irradiation depletes endogenous germ cells and increases donor cell distribution in chimeric chickens. / Park, Kyung Je; Kang, Seok Jin; Kim, Tae Min; Lee, Young Mok; Lee, Hyung Chul; Song, Gwonhwa; Han, Jae Yong.
In: In Vitro Cellular and Developmental Biology - Animal, Vol. 46, No. 10, 01.12.2010, p. 828-833.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gamma-irradiation depletes endogenous germ cells and increases donor cell distribution in chimeric chickens
AU - Park, Kyung Je
AU - Kang, Seok Jin
AU - Kim, Tae Min
AU - Lee, Young Mok
AU - Lee, Hyung Chul
AU - Song, Gwonhwa
AU - Han, Jae Yong
PY - 2010/12/1
Y1 - 2010/12/1
N2 - The production of chimeric birds is an important tool for the investigation of vertebrate development, the conservation of endangered birds, and the development of various biotechnological applications. This study examined whether gamma (γ)-irradiation depletes endogenous primordial germ cells and enhances the efficiency of somatic chimerism in chickens. An optimal irradiation protocol for stage X embryos was determined after irradiation at various doses (0, 100, 300, 500, 600, 700, and 2,000 rad). Exposure to 500 rad of γ-irradiation for 73 s significantly decreased the number of primordial germ cells (P∈<∈0.0001). Somatic chimera hatchlings were then produced by transferring blastodermal cells from a Korean Oge into either an irradiated (at 500 rad) or intact stage X White Leghorn embryo. An analysis of feather color pattern and polymerase chain reaction-based species-specific amplification of various tissues of the hatchlings confirmed chimerism in most organs of the chick produced from the irradiated recipient; a lesser degree of chimerism was observed in the non-irradiated control recipient. In conclusion, the exposure of chick embryos to an optimized dose of γ-irradiation effectively depleted germ cells and yielded greater somatic chimerism than non-irradiated control embryos. This technique can be applied to interspecies reproduction or the production of transgenic birds.
AB - The production of chimeric birds is an important tool for the investigation of vertebrate development, the conservation of endangered birds, and the development of various biotechnological applications. This study examined whether gamma (γ)-irradiation depletes endogenous primordial germ cells and enhances the efficiency of somatic chimerism in chickens. An optimal irradiation protocol for stage X embryos was determined after irradiation at various doses (0, 100, 300, 500, 600, 700, and 2,000 rad). Exposure to 500 rad of γ-irradiation for 73 s significantly decreased the number of primordial germ cells (P∈<∈0.0001). Somatic chimera hatchlings were then produced by transferring blastodermal cells from a Korean Oge into either an irradiated (at 500 rad) or intact stage X White Leghorn embryo. An analysis of feather color pattern and polymerase chain reaction-based species-specific amplification of various tissues of the hatchlings confirmed chimerism in most organs of the chick produced from the irradiated recipient; a lesser degree of chimerism was observed in the non-irradiated control recipient. In conclusion, the exposure of chick embryos to an optimized dose of γ-irradiation effectively depleted germ cells and yielded greater somatic chimerism than non-irradiated control embryos. This technique can be applied to interspecies reproduction or the production of transgenic birds.
KW - Chimera
KW - Gamma (γ)-irradiation
KW - Primordial germ cells (PGCs)
KW - Stage X embryos
UR - http://www.scopus.com/inward/record.url?scp=78751581563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78751581563&partnerID=8YFLogxK
U2 - 10.1007/s11626-010-9361-8
DO - 10.1007/s11626-010-9361-8
M3 - Article
C2 - 21057980
AN - SCOPUS:78751581563
VL - 46
SP - 828
EP - 833
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 10
ER -