Gastrin-releasing peptide (GRP) in the ovine uterus: Regulation by interferon tau and progesterone

Gwonhwa Song, M. Carey Satterfield, Jinyoung Kim, Fuller W. Bazer, Thomas E. Spencer

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Gastrin-releasing peptide (GRP) is abundantly expressed by endometrial glands of the ovine uterus and processed into different bioactive peptides, including GRP1-27, GRP18-27, and a C-terminus, that affect cell proliferation and migration. However, little information is available concerning the hormonal regulation of endometrial GRP and expression of GRP receptors in the ovine endometrium and conceptus. These studies determined the effects of pregnancy, progesterone (P4), interferon tau (IFNT), placental lactogen (CSH1), and growth hormone (GH) on expression of GRP in the endometrium and GRP receptors (GRPR, NMBR, BRS3) in the endometrium, conceptus, and placenta. In pregnant ewes, GRP mRNA and protein were first detected predominantly in endometrial glands after Day 10 and were abundant from Days 18 through 120 of gestation. Treatment with IFNT and progesterone but not CSH1 or GH stimulated GRP expression in the endometrial glands. Western blot analyses identified proGRP in uterine luminal fluid and allantoic fluid from Day 80 unilateral pregnant ewes but not in uterine luminal fluid of either cyclic or early pregnant ewes. GRPR mRNA was very low in the Day 18 conceptus and undetectable in the endometrium and placenta; NMBR and BRS3 mRNAs were undetectable in ovine uteroplacental tissues. Collectively, the present studies validate GRP as a novel IFNT-stimulated gene in the glands of the ovine uterus, revealed that IFNT induction of GRP is dependent on P4, and found that exposure of the ovine uterus to P4 for 20 days induces GRP expression in endometrial glands.

Original languageEnglish
Pages (from-to)376-386
Number of pages11
JournalBiology of Reproduction
Volume79
Issue number2
DOIs
Publication statusPublished - 2008 Aug 1
Externally publishedYes

Fingerprint

Gastrin-Releasing Peptide
Uterus
Progesterone
Sheep
Endometrium
Bombesin Receptors
Messenger RNA
Placenta
Growth Hormone
Placental Lactogen
Pregnancy
trophoblastin
Cell Movement
Western Blotting
Cell Proliferation
Peptides

Keywords

  • Gastrin-releasing peptide
  • Implantation
  • Interferon tau
  • Placenta
  • Pregnancy
  • Progesterone
  • Trophoblast
  • Uterus

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Gastrin-releasing peptide (GRP) in the ovine uterus : Regulation by interferon tau and progesterone. / Song, Gwonhwa; Satterfield, M. Carey; Kim, Jinyoung; Bazer, Fuller W.; Spencer, Thomas E.

In: Biology of Reproduction, Vol. 79, No. 2, 01.08.2008, p. 376-386.

Research output: Contribution to journalArticle

Song, Gwonhwa ; Satterfield, M. Carey ; Kim, Jinyoung ; Bazer, Fuller W. ; Spencer, Thomas E. / Gastrin-releasing peptide (GRP) in the ovine uterus : Regulation by interferon tau and progesterone. In: Biology of Reproduction. 2008 ; Vol. 79, No. 2. pp. 376-386.
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