Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation

Yoo Jin Joo, Jin Ha Kim, Un Beom Kang, Myeong Hee Yu, Joon Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Gcn4p is a well-characterized bZIP transcription factor that activates more than 500 genes encoding amino acids and purine biosynthesis enzymes, and many stress-response genes under various stress conditions. Under these stresses, it had been shown that transcriptions of ribosomal protein (RP) genes were decreased. However, the detailed mechanism of this downregulation has not been elucidated. In this study, we present a novel mechanistic model for a repressive role of Gcn4p on RP transcription, especially under amino-acid starvation. It was found that Gcn4p bound directly to Rap1p, which in turn inhibited Esa1p-Rap1p binding. The inhibition of Esa1p recruitment to RP promoters ultimately reduced the level of histone H4 acetylation and RP transcription. These data revealed that Gcn4p has simultaneous dual roles as a repressor for RP genes as well as an activator for amino-acid biosynthesis genes. Moreover, our results showed evidence of a novel link between general control of amino-acid biosynthesis and ribosome biogenesis mediated by Gcn4p at an early stage of adaptation to amino-acid starvation.

Original languageEnglish
Pages (from-to)859-872
Number of pages14
JournalEMBO Journal
Volume30
Issue number5
DOIs
Publication statusPublished - 2011 Mar 2

Fingerprint

Ribosomal Proteins
Starvation
Genes
Biosynthesis
Amino Acids
Transcription
Basic-Leucine Zipper Transcription Factors
Repressor Proteins
Acetylation
Gene encoding
Ribosomes
Histones
Down-Regulation
Enzymes

Keywords

  • amino-acid starvation
  • Gcn4p
  • Rap1p
  • ribosomal protein genes
  • transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation. / Joo, Yoo Jin; Kim, Jin Ha; Kang, Un Beom; Yu, Myeong Hee; Kim, Joon.

In: EMBO Journal, Vol. 30, No. 5, 02.03.2011, p. 859-872.

Research output: Contribution to journalArticle

Joo, Yoo Jin ; Kim, Jin Ha ; Kang, Un Beom ; Yu, Myeong Hee ; Kim, Joon. / Gcn4p-mediated transcriptional repression of ribosomal protein genes under amino-acid starvation. In: EMBO Journal. 2011 ; Vol. 30, No. 5. pp. 859-872.
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