GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper

Ki Jeong Kim, Jee Hyuck Lim, Min Jung Kim, Taesung Kim, Hyen Mi Chung, Kyung Hee Paek

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuum pathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation.

Original languageEnglish
Pages (from-to)693-698
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume374
Issue number4
DOIs
Publication statusPublished - 2008 Oct 3

Fingerprint

Capsicum
Lipase
Modulation
Wounds and Injuries
Viruses
Proteins
Tobacco
Tobacco Mosaic Virus
Genes
Salicylic Acid
Microarrays
Reverse Genetics
Complementary DNA
Gene Silencing
Microarray Analysis
Messenger RNA
Clone Cells
Gene Expression

Keywords

  • CaGL1
  • CaPR-4
  • Hot pepper (Capsicum annuum L.)
  • MeJA
  • TMV
  • Virus-induced gene silencing
  • Wound

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper. / Kim, Ki Jeong; Lim, Jee Hyuck; Kim, Min Jung; Kim, Taesung; Chung, Hyen Mi; Paek, Kyung Hee.

In: Biochemical and Biophysical Research Communications, Vol. 374, No. 4, 03.10.2008, p. 693-698.

Research output: Contribution to journalArticle

Kim, Ki Jeong ; Lim, Jee Hyuck ; Kim, Min Jung ; Kim, Taesung ; Chung, Hyen Mi ; Paek, Kyung Hee. / GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 374, No. 4. pp. 693-698.
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