Gemmobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat

Yeonjae Yoo, Dong Wan Lee, Hanbyul Lee, Bong Oh Kwon, Jong Seong Khim, Un Hyuk Yim, Hongjae Park, Byeonghyeok Park, In-Geol Choi, Beom Seok Kim, Seong Woo Jeon, Gyu Hyeok Kim, Jae-Jin Kim

Research output: Contribution to journalArticle

Abstract

A novel cream-pigmented marine bacterium, designated strain YJ-T1-11T, was isolated from a tidal flat at Yeongjong-do, Republic of Korea. Cells were rod-shaped, non-motile, aerobic, Gram-reaction-negative, oxidase-positive and catalase-positive. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain YJ-T1-11T clustered with Gemmobacter fontiphilus JS43T (98.3 %) within the genus Gemmobacter and its closest neighbours were G.emmobacter aquatilis DSM 3857T (98.5 %), Gemmobacter aquaticus A1-9T (98.4 %), Gemmobacterlanyuensis Orc-4T (98.4 %), Gemmobacterfontiphilus JS43T (98.3 %), Gemmobactercaeni DCA-1T (98.2 %), Gemmobacternanjingensis Y12T (97.5 %) and Gemmobactertilapiae Ruye-53T (97.2 %). Average nucleotide identity values between the genome sequences of strain YJ-T1-11T and the related type strains ranged from 77.08 to 90.48 %. The predominant fatty acid of strain YJ-T1-11T was summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c). The major isoprenoid quinone was Q-10 and the DNA G+C content was 65.6 mol%. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and three unidentified lipids. The DNA-DNA relatedness values between strain YJ-T1-11T and the type strains of the 12 phylogenetically related species of the genus Gemmobacter were 23.6-53.7 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain YJ-T1-11T is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter lutimaris sp. nov. is proposed. The type strain is YJ-T1-11T (=KCTC 62715T=JCM 32828T).

Original languageEnglish
Pages (from-to)1676-1681
Number of pages6
JournalInternational journal of systematic and evolutionary microbiology
Volume69
Issue number6
DOIs
Publication statusPublished - 2019 Jun 1

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Gemmobacter
tidal flat
Bacteria
DNA
bacterium
new species
bacteria
lipid
Lipids
Republic of Korea
Phosphatidylglycerols
Cardiolipins
isoprenoid
Terpenes
Phosphatidylcholines
rRNA Genes
relatedness
Catalase
Names
Oxidoreductases

Keywords

  • Gemmobacter
  • polyphasic approach
  • Proteobacteria
  • tidal flat

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Gemmobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat. / Yoo, Yeonjae; Lee, Dong Wan; Lee, Hanbyul; Kwon, Bong Oh; Khim, Jong Seong; Yim, Un Hyuk; Park, Hongjae; Park, Byeonghyeok; Choi, In-Geol; Kim, Beom Seok; Jeon, Seong Woo; Kim, Gyu Hyeok; Kim, Jae-Jin.

In: International journal of systematic and evolutionary microbiology, Vol. 69, No. 6, 01.06.2019, p. 1676-1681.

Research output: Contribution to journalArticle

Yoo, Yeonjae ; Lee, Dong Wan ; Lee, Hanbyul ; Kwon, Bong Oh ; Khim, Jong Seong ; Yim, Un Hyuk ; Park, Hongjae ; Park, Byeonghyeok ; Choi, In-Geol ; Kim, Beom Seok ; Jeon, Seong Woo ; Kim, Gyu Hyeok ; Kim, Jae-Jin. / Gemmobacter lutimaris sp. nov., a marine bacterium isolated from a tidal flat. In: International journal of systematic and evolutionary microbiology. 2019 ; Vol. 69, No. 6. pp. 1676-1681.
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abstract = "A novel cream-pigmented marine bacterium, designated strain YJ-T1-11T, was isolated from a tidal flat at Yeongjong-do, Republic of Korea. Cells were rod-shaped, non-motile, aerobic, Gram-reaction-negative, oxidase-positive and catalase-positive. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain YJ-T1-11T clustered with Gemmobacter fontiphilus JS43T (98.3 {\%}) within the genus Gemmobacter and its closest neighbours were G.emmobacter aquatilis DSM 3857T (98.5 {\%}), Gemmobacter aquaticus A1-9T (98.4 {\%}), Gemmobacterlanyuensis Orc-4T (98.4 {\%}), Gemmobacterfontiphilus JS43T (98.3 {\%}), Gemmobactercaeni DCA-1T (98.2 {\%}), Gemmobacternanjingensis Y12T (97.5 {\%}) and Gemmobactertilapiae Ruye-53T (97.2 {\%}). Average nucleotide identity values between the genome sequences of strain YJ-T1-11T and the related type strains ranged from 77.08 to 90.48 {\%}. The predominant fatty acid of strain YJ-T1-11T was summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c). The major isoprenoid quinone was Q-10 and the DNA G+C content was 65.6 mol{\%}. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and three unidentified lipids. The DNA-DNA relatedness values between strain YJ-T1-11T and the type strains of the 12 phylogenetically related species of the genus Gemmobacter were 23.6-53.7 {\%}. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain YJ-T1-11T is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter lutimaris sp. nov. is proposed. The type strain is YJ-T1-11T (=KCTC 62715T=JCM 32828T).",
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AU - Choi, In-Geol

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N2 - A novel cream-pigmented marine bacterium, designated strain YJ-T1-11T, was isolated from a tidal flat at Yeongjong-do, Republic of Korea. Cells were rod-shaped, non-motile, aerobic, Gram-reaction-negative, oxidase-positive and catalase-positive. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain YJ-T1-11T clustered with Gemmobacter fontiphilus JS43T (98.3 %) within the genus Gemmobacter and its closest neighbours were G.emmobacter aquatilis DSM 3857T (98.5 %), Gemmobacter aquaticus A1-9T (98.4 %), Gemmobacterlanyuensis Orc-4T (98.4 %), Gemmobacterfontiphilus JS43T (98.3 %), Gemmobactercaeni DCA-1T (98.2 %), Gemmobacternanjingensis Y12T (97.5 %) and Gemmobactertilapiae Ruye-53T (97.2 %). Average nucleotide identity values between the genome sequences of strain YJ-T1-11T and the related type strains ranged from 77.08 to 90.48 %. The predominant fatty acid of strain YJ-T1-11T was summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c). The major isoprenoid quinone was Q-10 and the DNA G+C content was 65.6 mol%. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and three unidentified lipids. The DNA-DNA relatedness values between strain YJ-T1-11T and the type strains of the 12 phylogenetically related species of the genus Gemmobacter were 23.6-53.7 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain YJ-T1-11T is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter lutimaris sp. nov. is proposed. The type strain is YJ-T1-11T (=KCTC 62715T=JCM 32828T).

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