Gene expression analysis reveals a functional role for the Ag-NPs-induced Egr-1 transcriptional factor in human keratinocytes

Gyeongmin Yoo, Sang Hoon Jeong, Woo In Ryu, Hana Lee, Jin Hee Kim, Hyun Cheol Bae, Sang Wook Son

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Silver nanoparticles (Ag-NPs) have wellknown antimicrobial properties and have been widely applied as nano-strategies in myriad medical fields. Indeed, early growth response (Egr-1) is an important transcription factor that has been implicated in numerous inflammatory diseases. The administration of Ag-NPs transiently increased the levels of Egr-1 mRNA in human keratinocytes with maximal induction at the 30 minute time point. In an effort to identify molecular signatures associated with cellular responses to Ag-NPs, we adopted an approach involving gene expression profiling of human keratinocytes treated with or without Egr-1 siRNA transfection using a cDNA microarray. The microarray analysis demonstrated that: the expression profile of the genes involved in inflammation and immune response were either stimulated or repressed. Overall, this study was sufficient to reliably recognize the engagement of Egr-1-driven molecular signaling pathways in Ag-NPs-treated keratinocytes.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalMolecular and Cellular Toxicology
Volume10
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

Keratinocytes
Gene expression
Microarrays
Gene Expression
Gene Expression Profiling
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Transcriptome
Silver
Nanoparticles
Small Interfering RNA
Transfection
Transcription Factors
Complementary DNA
Genes
Inflammation
Messenger RNA
Growth

Keywords

  • Early growth response-1
  • Keratinocytes
  • Microarray
  • Silver nanoparticles

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Pathology and Forensic Medicine

Cite this

Gene expression analysis reveals a functional role for the Ag-NPs-induced Egr-1 transcriptional factor in human keratinocytes. / Yoo, Gyeongmin; Jeong, Sang Hoon; Ryu, Woo In; Lee, Hana; Kim, Jin Hee; Bae, Hyun Cheol; Son, Sang Wook.

In: Molecular and Cellular Toxicology, Vol. 10, No. 2, 2014, p. 149-156.

Research output: Contribution to journalArticle

Yoo, Gyeongmin ; Jeong, Sang Hoon ; Ryu, Woo In ; Lee, Hana ; Kim, Jin Hee ; Bae, Hyun Cheol ; Son, Sang Wook. / Gene expression analysis reveals a functional role for the Ag-NPs-induced Egr-1 transcriptional factor in human keratinocytes. In: Molecular and Cellular Toxicology. 2014 ; Vol. 10, No. 2. pp. 149-156.
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