Gene expression analysis reveals a functional role for the Ag-NPs-induced Egr-1 transcriptional factor in human keratinocytes

Gyeongmin Yoo, Sang Hoon Jeong, Woo In Ryu, Hana Lee, Jin Hee Kim, Hyun Cheol Bae, Sang Wook Son

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Silver nanoparticles (Ag-NPs) have wellknown antimicrobial properties and have been widely applied as nano-strategies in myriad medical fields. Indeed, early growth response (Egr-1) is an important transcription factor that has been implicated in numerous inflammatory diseases. The administration of Ag-NPs transiently increased the levels of Egr-1 mRNA in human keratinocytes with maximal induction at the 30 minute time point. In an effort to identify molecular signatures associated with cellular responses to Ag-NPs, we adopted an approach involving gene expression profiling of human keratinocytes treated with or without Egr-1 siRNA transfection using a cDNA microarray. The microarray analysis demonstrated that: the expression profile of the genes involved in inflammation and immune response were either stimulated or repressed. Overall, this study was sufficient to reliably recognize the engagement of Egr-1-driven molecular signaling pathways in Ag-NPs-treated keratinocytes.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalMolecular and Cellular Toxicology
Volume10
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • Early growth response-1
  • Keratinocytes
  • Microarray
  • Silver nanoparticles

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Pathology and Forensic Medicine

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