Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell

Hyun Kim Dong, Hee Yoo Keon, Suk Choi Kyung, Jaewon Choi, Sang Yun Choi, Sung Eun Yang, Yoon Sun Yang, Joon Im Ho, Hyun Kim Kye, Lim Jung Hye, Woong Sung Ki, Hoe Koo Hong

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs), which are adherent stromal cells of a nonhematopoietic origin, have the ability to give rise to various differentiated cell types. MSCs regulate localization, self-renewal and differentiation of hematopoietic stem cells (HSCs) due to MSCs' secretion of cytokines and growth factors, the cell-to-cell interactions and the influence of the extracellular matrix proteins. Using RT-PCR analysis, we examined the expression levels of cytokines and growth factors from MSCs and their differentiated cell types, including osteoblasts, adipocytes and endothelial cells. Cytokine and growth factor genes, including IL-6, IL-8, IL-11, IL-12, IL-14, IL-15, LIF, G-CSF, GM-CSF, M-SCF, FL and SCF, were found to be expressed in the MSCs. In contrast, there was no IL-1α, IL-1β, or IL-7 expression observed. The IL-12, IL-14, G-CSF, and GM-CSF mRNA expression levels either disappeared or decreased after the MSCs differentiated into osteoblasts, adipocytes, and endothelial cells. Among the differentiated cells derived from MSCs, osteoblasts, adipocytes, and endothelial cells expressed the osteopontin, aP2, and the VEGFR-2 gene, respectively. These profiles could help determine future clinical applications of MSCs and their derivatives for cell therapy.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalCytokine
Volume31
Issue number2
DOIs
Publication statusPublished - 2005 Jul 21

Keywords

  • Cytokines
  • Growth factors
  • Mesenchymal stem cells
  • Reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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  • Cite this

    Dong, H. K., Keon, H. Y., Kyung, S. C., Choi, J., Choi, S. Y., Yang, S. E., Yang, Y. S., Ho, J. I., Kye, H. K., Hye, L. J., Ki, W. S., & Hong, H. K. (2005). Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell. Cytokine, 31(2), 119-126. https://doi.org/10.1016/j.cyto.2005.04.004