Abstract
Mesenchymal stem cells (MSCs), which are adherent stromal cells of a nonhematopoietic origin, have the ability to give rise to various differentiated cell types. MSCs regulate localization, self-renewal and differentiation of hematopoietic stem cells (HSCs) due to MSCs' secretion of cytokines and growth factors, the cell-to-cell interactions and the influence of the extracellular matrix proteins. Using RT-PCR analysis, we examined the expression levels of cytokines and growth factors from MSCs and their differentiated cell types, including osteoblasts, adipocytes and endothelial cells. Cytokine and growth factor genes, including IL-6, IL-8, IL-11, IL-12, IL-14, IL-15, LIF, G-CSF, GM-CSF, M-SCF, FL and SCF, were found to be expressed in the MSCs. In contrast, there was no IL-1α, IL-1β, or IL-7 expression observed. The IL-12, IL-14, G-CSF, and GM-CSF mRNA expression levels either disappeared or decreased after the MSCs differentiated into osteoblasts, adipocytes, and endothelial cells. Among the differentiated cells derived from MSCs, osteoblasts, adipocytes, and endothelial cells expressed the osteopontin, aP2, and the VEGFR-2 gene, respectively. These profiles could help determine future clinical applications of MSCs and their derivatives for cell therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 119-126 |
| Number of pages | 8 |
| Journal | Cytokine |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 Jul 21 |
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Keywords
- Cytokines
- Growth factors
- Mesenchymal stem cells
- Reverse transcriptase polymerase chain reaction
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Immunology
- Immunology and Allergy
Cite this
Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell. / Dong, Hyun Kim; Keon, Hee Yoo; Kyung, Suk Choi; Choi, Jaewon; Choi, Sang-Yun; Yang, Sung Eun; Yang, Yoon Sun; Ho, Joon Im; Kye, Hyun Kim; Hye, Lim Jung; Ki, Woong Sung; Hong, Hoe Koo.
In: Cytokine, Vol. 31, No. 2, 21.07.2005, p. 119-126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell
AU - Dong, Hyun Kim
AU - Keon, Hee Yoo
AU - Kyung, Suk Choi
AU - Choi, Jaewon
AU - Choi, Sang-Yun
AU - Yang, Sung Eun
AU - Yang, Yoon Sun
AU - Ho, Joon Im
AU - Kye, Hyun Kim
AU - Hye, Lim Jung
AU - Ki, Woong Sung
AU - Hong, Hoe Koo
PY - 2005/7/21
Y1 - 2005/7/21
N2 - Mesenchymal stem cells (MSCs), which are adherent stromal cells of a nonhematopoietic origin, have the ability to give rise to various differentiated cell types. MSCs regulate localization, self-renewal and differentiation of hematopoietic stem cells (HSCs) due to MSCs' secretion of cytokines and growth factors, the cell-to-cell interactions and the influence of the extracellular matrix proteins. Using RT-PCR analysis, we examined the expression levels of cytokines and growth factors from MSCs and their differentiated cell types, including osteoblasts, adipocytes and endothelial cells. Cytokine and growth factor genes, including IL-6, IL-8, IL-11, IL-12, IL-14, IL-15, LIF, G-CSF, GM-CSF, M-SCF, FL and SCF, were found to be expressed in the MSCs. In contrast, there was no IL-1α, IL-1β, or IL-7 expression observed. The IL-12, IL-14, G-CSF, and GM-CSF mRNA expression levels either disappeared or decreased after the MSCs differentiated into osteoblasts, adipocytes, and endothelial cells. Among the differentiated cells derived from MSCs, osteoblasts, adipocytes, and endothelial cells expressed the osteopontin, aP2, and the VEGFR-2 gene, respectively. These profiles could help determine future clinical applications of MSCs and their derivatives for cell therapy.
AB - Mesenchymal stem cells (MSCs), which are adherent stromal cells of a nonhematopoietic origin, have the ability to give rise to various differentiated cell types. MSCs regulate localization, self-renewal and differentiation of hematopoietic stem cells (HSCs) due to MSCs' secretion of cytokines and growth factors, the cell-to-cell interactions and the influence of the extracellular matrix proteins. Using RT-PCR analysis, we examined the expression levels of cytokines and growth factors from MSCs and their differentiated cell types, including osteoblasts, adipocytes and endothelial cells. Cytokine and growth factor genes, including IL-6, IL-8, IL-11, IL-12, IL-14, IL-15, LIF, G-CSF, GM-CSF, M-SCF, FL and SCF, were found to be expressed in the MSCs. In contrast, there was no IL-1α, IL-1β, or IL-7 expression observed. The IL-12, IL-14, G-CSF, and GM-CSF mRNA expression levels either disappeared or decreased after the MSCs differentiated into osteoblasts, adipocytes, and endothelial cells. Among the differentiated cells derived from MSCs, osteoblasts, adipocytes, and endothelial cells expressed the osteopontin, aP2, and the VEGFR-2 gene, respectively. These profiles could help determine future clinical applications of MSCs and their derivatives for cell therapy.
KW - Cytokines
KW - Growth factors
KW - Mesenchymal stem cells
KW - Reverse transcriptase polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=21344471489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21344471489&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2005.04.004
DO - 10.1016/j.cyto.2005.04.004
M3 - Article
VL - 31
SP - 119
EP - 126
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 2
ER -