TY - JOUR
T1 - Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell
AU - Dong, Hyun Kim
AU - Keon, Hee Yoo
AU - Kyung, Suk Choi
AU - Choi, Jaewon
AU - Choi, Sang Yun
AU - Yang, Sung Eun
AU - Yang, Yoon Sun
AU - Ho, Joon Im
AU - Kye, Hyun Kim
AU - Hye, Lim Jung
AU - Ki, Woong Sung
AU - Hong, Hoe Koo
N1 - Funding Information:
This study was supported by a grant from Samsung Biomedical Research Institute grant, #SBRI C-A5-203-1 and the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (0405-DB000-0101-0016).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/7/21
Y1 - 2005/7/21
N2 - Mesenchymal stem cells (MSCs), which are adherent stromal cells of a nonhematopoietic origin, have the ability to give rise to various differentiated cell types. MSCs regulate localization, self-renewal and differentiation of hematopoietic stem cells (HSCs) due to MSCs' secretion of cytokines and growth factors, the cell-to-cell interactions and the influence of the extracellular matrix proteins. Using RT-PCR analysis, we examined the expression levels of cytokines and growth factors from MSCs and their differentiated cell types, including osteoblasts, adipocytes and endothelial cells. Cytokine and growth factor genes, including IL-6, IL-8, IL-11, IL-12, IL-14, IL-15, LIF, G-CSF, GM-CSF, M-SCF, FL and SCF, were found to be expressed in the MSCs. In contrast, there was no IL-1α, IL-1β, or IL-7 expression observed. The IL-12, IL-14, G-CSF, and GM-CSF mRNA expression levels either disappeared or decreased after the MSCs differentiated into osteoblasts, adipocytes, and endothelial cells. Among the differentiated cells derived from MSCs, osteoblasts, adipocytes, and endothelial cells expressed the osteopontin, aP2, and the VEGFR-2 gene, respectively. These profiles could help determine future clinical applications of MSCs and their derivatives for cell therapy.
AB - Mesenchymal stem cells (MSCs), which are adherent stromal cells of a nonhematopoietic origin, have the ability to give rise to various differentiated cell types. MSCs regulate localization, self-renewal and differentiation of hematopoietic stem cells (HSCs) due to MSCs' secretion of cytokines and growth factors, the cell-to-cell interactions and the influence of the extracellular matrix proteins. Using RT-PCR analysis, we examined the expression levels of cytokines and growth factors from MSCs and their differentiated cell types, including osteoblasts, adipocytes and endothelial cells. Cytokine and growth factor genes, including IL-6, IL-8, IL-11, IL-12, IL-14, IL-15, LIF, G-CSF, GM-CSF, M-SCF, FL and SCF, were found to be expressed in the MSCs. In contrast, there was no IL-1α, IL-1β, or IL-7 expression observed. The IL-12, IL-14, G-CSF, and GM-CSF mRNA expression levels either disappeared or decreased after the MSCs differentiated into osteoblasts, adipocytes, and endothelial cells. Among the differentiated cells derived from MSCs, osteoblasts, adipocytes, and endothelial cells expressed the osteopontin, aP2, and the VEGFR-2 gene, respectively. These profiles could help determine future clinical applications of MSCs and their derivatives for cell therapy.
KW - Cytokines
KW - Growth factors
KW - Mesenchymal stem cells
KW - Reverse transcriptase polymerase chain reaction
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U2 - 10.1016/j.cyto.2005.04.004
DO - 10.1016/j.cyto.2005.04.004
M3 - Article
C2 - 15919213
AN - SCOPUS:21344471489
VL - 31
SP - 119
EP - 126
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 2
ER -