Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell

Hyun Kim Dong, Hee Yoo Keon, Suk Choi Kyung, Jaewon Choi, Sang-Yun Choi, Sung Eun Yang, Yoon Sun Yang, Joon Im Ho, Hyun Kim Kye, Lim Jung Hye, Woong Sung Ki, Hoe Koo Hong

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs), which are adherent stromal cells of a nonhematopoietic origin, have the ability to give rise to various differentiated cell types. MSCs regulate localization, self-renewal and differentiation of hematopoietic stem cells (HSCs) due to MSCs' secretion of cytokines and growth factors, the cell-to-cell interactions and the influence of the extracellular matrix proteins. Using RT-PCR analysis, we examined the expression levels of cytokines and growth factors from MSCs and their differentiated cell types, including osteoblasts, adipocytes and endothelial cells. Cytokine and growth factor genes, including IL-6, IL-8, IL-11, IL-12, IL-14, IL-15, LIF, G-CSF, GM-CSF, M-SCF, FL and SCF, were found to be expressed in the MSCs. In contrast, there was no IL-1α, IL-1β, or IL-7 expression observed. The IL-12, IL-14, G-CSF, and GM-CSF mRNA expression levels either disappeared or decreased after the MSCs differentiated into osteoblasts, adipocytes, and endothelial cells. Among the differentiated cells derived from MSCs, osteoblasts, adipocytes, and endothelial cells expressed the osteopontin, aP2, and the VEGFR-2 gene, respectively. These profiles could help determine future clinical applications of MSCs and their derivatives for cell therapy.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalCytokine
Volume31
Issue number2
DOIs
Publication statusPublished - 2005 Jul 21

Fingerprint

Growth Differentiation Factors
Stem cells
Mesenchymal Stromal Cells
Transcriptome
Gene expression
Intercellular Signaling Peptides and Proteins
Bone
Bone Marrow
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Osteoblasts
Endothelial cells
Adipocytes
Endothelial Cells
Granulocyte Colony-Stimulating Factor
Interleukin-12
Interleukin-1
Interleukin-11
Genes
Interleukin-15

Keywords

  • Cytokines
  • Growth factors
  • Mesenchymal stem cells
  • Reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell. / Dong, Hyun Kim; Keon, Hee Yoo; Kyung, Suk Choi; Choi, Jaewon; Choi, Sang-Yun; Yang, Sung Eun; Yang, Yoon Sun; Ho, Joon Im; Kye, Hyun Kim; Hye, Lim Jung; Ki, Woong Sung; Hong, Hoe Koo.

In: Cytokine, Vol. 31, No. 2, 21.07.2005, p. 119-126.

Research output: Contribution to journalArticle

Dong, HK, Keon, HY, Kyung, SC, Choi, J, Choi, S-Y, Yang, SE, Yang, YS, Ho, JI, Kye, HK, Hye, LJ, Ki, WS & Hong, HK 2005, 'Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell', Cytokine, vol. 31, no. 2, pp. 119-126. https://doi.org/10.1016/j.cyto.2005.04.004
Dong, Hyun Kim ; Keon, Hee Yoo ; Kyung, Suk Choi ; Choi, Jaewon ; Choi, Sang-Yun ; Yang, Sung Eun ; Yang, Yoon Sun ; Ho, Joon Im ; Kye, Hyun Kim ; Hye, Lim Jung ; Ki, Woong Sung ; Hong, Hoe Koo. / Gene expression profile of cytokine and growth factor during differentiation of bone marrow-derived mesenchymal stem cell. In: Cytokine. 2005 ; Vol. 31, No. 2. pp. 119-126.
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