Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae

Mukesh Kumar Yadav, Seong Keun Kwon, Chang Gun Cho, Seok Won Park, Sungwon Chae, Jae-Jun Song

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study, the gene expression profile of early in vitro Streptococcus pneumoniae biofilm with respect to planktonic cells in cDNA microarray analysis is reported. Microarray analysis with respect to planktonic cells was performed on total RNA extracted from biofilms grown in 24-well microtiter plates. To validate the microarray results, real-time RT-PCR was performed on 13 differentially expressed genes and one constitutively expressed gene. The cDNA-microarray analyses identified 89 genes that were significantly differentially expressed in biofilm and planktonic cells. Genes involved in isoprenoid biosynthesis, cell wall biosynthesis, translation and purine and pyrimidine nucleotide metabolic pathways were exclusively expressed in the biofilms, whereas transcription regulator genes were exclusively expressed in planktonic cells. The real-time RT-PCR results of 13 differentially regulated genes were completely in agreement with the microarray data. The exclusive up regulation in biofilms of genes involved in the mevalonate pathway, cell wall biosynthesis, translation and purine and pyrimidine nucleotide metabolic pathways suggests that expression of these genes may be required for initial biofilm formation, and growth and survival of bacteria in biofilms. The up regulation of related genes suggests that cells in biofilms may be under stress conditions and possibly actively involved in the protein synthesis required to adapt to a new environment.

Original languageEnglish
Pages (from-to)621-629
Number of pages9
JournalMicrobiology and Immunology
Volume56
Issue number9
DOIs
Publication statusPublished - 2012 Sep 1

Fingerprint

Biofilms
Streptococcus pneumoniae
Transcriptome
Genes
Microarray Analysis
Pyrimidine Nucleotides
Purine Nucleotides
Metabolic Networks and Pathways
Oligonucleotide Array Sequence Analysis
Cell Wall
Real-Time Polymerase Chain Reaction
Up-Regulation
Mevalonic Acid
In Vitro Techniques
Terpenes
Regulator Genes
RNA
Bacteria
Gene Expression
Growth

Keywords

  • Biofilm
  • Gene expression
  • Microarray
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology

Cite this

Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae. / Yadav, Mukesh Kumar; Kwon, Seong Keun; Cho, Chang Gun; Park, Seok Won; Chae, Sungwon; Song, Jae-Jun.

In: Microbiology and Immunology, Vol. 56, No. 9, 01.09.2012, p. 621-629.

Research output: Contribution to journalArticle

Yadav, Mukesh Kumar ; Kwon, Seong Keun ; Cho, Chang Gun ; Park, Seok Won ; Chae, Sungwon ; Song, Jae-Jun. / Gene expression profile of early in vitro biofilms of Streptococcus pneumoniae. In: Microbiology and Immunology. 2012 ; Vol. 56, No. 9. pp. 621-629.
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