Gene expression profiles during the activation of rat hepatic stellate cells evaluated by cDNA microarray

Wook Woo Sun, Kwan Ik Hwang, Myeon Woo Chung, Kyung Jin Sun, Syrie Bang, Sung Ho Lee, Hee Lee Sung, Joo Chung Hye, Hwan Sohn Dong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hepatic stellate cells (HSCs) are activated by producing potentially injurious connective tissue components during hepatic fibrosis, thereby exerting a pivotal action in the development of liver fibrogenesis. The aim of this study was to investigate differences in gene expression patterns during the activation of HSCs using complementary cDNA microarrays. HSCs were isolated from normal rat livers and cultured for 0 (3 h), 3, 5 and 7 d. RNA was extracted from cultured cells at each point. The target RNA was hybridized to gene-specific sequence probes immobilized on chips. The hybridization signal was assessed using a confocal laser scanner. Comparison of hybridization signals and patterns allows the identification of mRNAs that are expressed differentially. Statistical analysis was used to classify and cluster the genes according to their up- or downregulation. As a result, 33 upregulated early-stage and 36 upregulated late-stage gene candidates were identified. This time-based study revealed a number of newly discovered genes involved in fibrogenesis during the activation of HSCs.

Original languageEnglish
Pages (from-to)1410-1418
Number of pages9
JournalArchives of Pharmacal Research
Volume30
Issue number11
Publication statusPublished - 2007 Nov 30

Keywords

  • DNA chip
  • DNA microarray
  • Gene expression
  • Hepatic fibrosis
  • Hepatic stellate cell

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Gene expression profiles during the activation of rat hepatic stellate cells evaluated by cDNA microarray'. Together they form a unique fingerprint.

  • Cite this

    Sun, W. W., Hwang, K. I., Chung, M. W., Sun, K. J., Bang, S., Lee, S. H., Sung, H. L., Hye, J. C., & Dong, H. S. (2007). Gene expression profiles during the activation of rat hepatic stellate cells evaluated by cDNA microarray. Archives of Pharmacal Research, 30(11), 1410-1418.