TY - JOUR
T1 - Gene Expression Profiles of Papillary Thyroid Microcarcinoma
AU - Kim, Woo Young
AU - Lee, Jae Bok
AU - Jung, Seung Pil
AU - Kim, Hoon Yub
AU - Woo, Sang Uk
AU - Son, Gil Soo
AU - Bae, Jeoung Won
N1 - Funding Information:
The authors report no declarations of interest. This study was sponsored by Korea University Grants.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The objective was to identify gene expression profile of papillary thyroid microcarcinoma. To help improve diagnosis of papillary thyroid microcarcinoma, we performed gene expression profiling and compared it to pair normal thyroid tissues. We performed microarray analysis with 6 papillary thyroid microcarcinoma and 6 pair normal thyroid tissues. Differentially expressed genes were selected using paired t test, linear models for microarray data, and significance analysis of microarrays. Real-time quantitative reverse transcription–polymerase chain reaction was used to validate the representative 10 genes (MET, TIMP1, QPCT, PROS1, LRP4, SDC4, CITED1, DPP4, LRRK2, RUNX2). We identified 91 differentially expressed genes (84 upregulated and 7 downregulated) in the gene expression profile and validated 10 genes of the profile. We identified a significant genetic difference between papillary thyroid microcarcinoma and normal tissue by 10 upregulated genes greater than 2-fold (P, 0.05).
AB - The objective was to identify gene expression profile of papillary thyroid microcarcinoma. To help improve diagnosis of papillary thyroid microcarcinoma, we performed gene expression profiling and compared it to pair normal thyroid tissues. We performed microarray analysis with 6 papillary thyroid microcarcinoma and 6 pair normal thyroid tissues. Differentially expressed genes were selected using paired t test, linear models for microarray data, and significance analysis of microarrays. Real-time quantitative reverse transcription–polymerase chain reaction was used to validate the representative 10 genes (MET, TIMP1, QPCT, PROS1, LRP4, SDC4, CITED1, DPP4, LRRK2, RUNX2). We identified 91 differentially expressed genes (84 upregulated and 7 downregulated) in the gene expression profile and validated 10 genes of the profile. We identified a significant genetic difference between papillary thyroid microcarcinoma and normal tissue by 10 upregulated genes greater than 2-fold (P, 0.05).
KW - Gene expression profile
KW - Microarray analysis
KW - Papillary thyroid microcarcinoma
UR - http://www.scopus.com/inward/record.url?scp=85041579039&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85041579039&partnerID=8YFLogxK
U2 - 10.9738/INTSURG-D-17-00049.1
DO - 10.9738/INTSURG-D-17-00049.1
M3 - Article
AN - SCOPUS:85041579039
VL - 102
SP - 39
EP - 46
JO - International Surgery
JF - International Surgery
SN - 0020-8868
IS - 1-2
ER -