Gene expression profiles of rat olfactory bulb at developmental stage

Jae Hoon Cho, Hak Hyun Jung, Sang Hag Lee, Gi Jung Im, Jiwon Chang, Seo Jin Kim

Research output: Contribution to journalArticle

Abstract

Microarray analysis may be a useful tool to identify some candidate genes related to the development of olfactory bulbs. In the present study, gene expression profiles of olfactory bulbs from postnatal day 1 (P1) rats and postnatal day 35 (P35) rats were analyzed by oligonucleotide-microarray and expression levels of some selected genes were also confirmed by RT-PCR and in situ hybridization. 9146 genes were commonly identified in six microarray chips. Among these genes, 76 were up-regulated and 130 were down-regulated three-folds or more at P1 olfactory bulbs. Out of these 76 up-regulated genes, 24 genes were annotated based on the NCBI database of reference sequences and expression levels of these 24 genes were confirmed by RT-PCR. Among them, 2 interesting genes (neurogenic differentiation 1 and retinoid acid receptor alpha) were localized in the P1 olfactory bulb by the use of in situ hybridization technique. Our results may provide basic information to identify genes associated with functional growth of olfactory bulbs.

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalJournal of Neuroscience Methods
Volume177
Issue number1
DOIs
Publication statusPublished - 2009 Feb 15

Fingerprint

Olfactory Bulb
Transcriptome
Genes
In Situ Hybridization
Polymerase Chain Reaction
Retinoids
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Databases
Acids
Growth

Keywords

  • Development
  • Gene expression
  • Olfactory bulb
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Gene expression profiles of rat olfactory bulb at developmental stage. / Cho, Jae Hoon; Jung, Hak Hyun; Lee, Sang Hag; Im, Gi Jung; Chang, Jiwon; Kim, Seo Jin.

In: Journal of Neuroscience Methods, Vol. 177, No. 1, 15.02.2009, p. 14-19.

Research output: Contribution to journalArticle

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