Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice

Jayalakshmi Krishnan, Jiayan Chen, Kum Joo Shin, Jong-Ik Hwang, Sang Uk Han, Gwang Lee, Sangdun Choi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Exposure to light can induce photoreceptor cell death and exacerbate retinal degeneration. In this study, mice with genetic knockout of several genes, including rhodopsin kinase (Rhok -/-), arrestin (Sag -/-), transducin (Gnat1 -/-), c-Fos (c-Fos -/-) and arrestin/transducin (Sag -/-/Gnat1 -/-), were examined. We measured the expression levels of thousands of genes in order to investigate their roles in phototransduction signaling in light-induced retinal degeneration using DNA microarray technology and then further explored the gene network using pathway analysis tools. Several cascades of gene components were induced or inhibited as a result of corresponding gene knockout under specific light conditions. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. Deletion of c-Fos blocked the apoptotic signaling induced by exposure to high intensity light. In the present study, we identified many gene transcripts that are essential for the initiation of light-induced rod degeneration and proposed several important networks that are involved in pro- and anti-apoptotic signaling. We also demonstrated the different cascades of gene components that participate in apoptotic signaling under specific light conditions.

Original languageEnglish
Pages (from-to)495-504
Number of pages10
JournalExperimental and Molecular Medicine
Volume40
Issue number5
DOIs
Publication statusPublished - 2008 Oct 1

Fingerprint

Light Signal Transduction
Retinal Degeneration
Gene Knockout Techniques
Gene Expression Profiling
Gene expression
Knockout Mice
Genes
Light
Transducin
Gene Components
Arrestin
G-Protein-Coupled Receptor Kinase 1
High intensity light
Photoreceptor Cells
Gene Order
Gene Regulatory Networks
Essential Genes
Transcription Factor AP-1
Cell death
Microarrays

Keywords

  • Arrestin
  • Gene expression profiling
  • Oligonucleotide array sequence analysis
  • Proto-oncogene proteins c-fos
  • Retinal degeneration
  • Transducin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Clinical Biochemistry

Cite this

Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice. / Krishnan, Jayalakshmi; Chen, Jiayan; Shin, Kum Joo; Hwang, Jong-Ik; Han, Sang Uk; Lee, Gwang; Choi, Sangdun.

In: Experimental and Molecular Medicine, Vol. 40, No. 5, 01.10.2008, p. 495-504.

Research output: Contribution to journalArticle

Krishnan, Jayalakshmi ; Chen, Jiayan ; Shin, Kum Joo ; Hwang, Jong-Ik ; Han, Sang Uk ; Lee, Gwang ; Choi, Sangdun. / Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice. In: Experimental and Molecular Medicine. 2008 ; Vol. 40, No. 5. pp. 495-504.
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