Gene profiles during vestibular compensation in rats after unilateral labyrinthectomy

Moo Kyun Park, Byung Don Lee, Jong Dae Lee, Hak Hyun Jung, Sung Won Chae

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Objectives: The aims of this study were to determine changes in gene expression in the chronic state of vestibular compensation by microarray analysis and to validate the asymmetrical levels of gene expression in the ipsilateral and contralateral vestibular nucleus complexes (VNCs). Methods: Microarray analysis was used to examine the expression of genes up-regulated or down-regulated in the ipsilateral VNC at 1 and 7 days after unilateral labyrinthectomy. Up-regulated or down-regulated gene expression in the ipsilateral and contralateral VNCs was then validated by reverse transcriptase polymerase chain reaction at 1, 7, 14, and 28 days after labyrinthectomy. Results: The genes down-regulated at 1 day after labyrinthectomy and up-regulated at 7 days after labyrinthectomy as determined by microarray analysis and reverse transcriptase polymerase chain reaction were zinc finger protein 307, zinc metallopeptidase, P34, calcitonin receptor, insulin-like growth factor binding protein 5, GATA binding protein 3, and CD151. Expression of zinc finger protein 307, zinc metallopeptidase, P34, and calcitonin receptor was up-regulated even after 7 days in the contralateral VNC of rats that had labyrinthectomy. Conclusions: This study demonstrated changes in gene expression in rats during the chronic phase of vestibular compensation after unilateral labyrinthectomy and provided profiles of these changes in gene expression.

    Original languageEnglish
    Pages (from-to)761-769
    Number of pages9
    JournalAnnals of Otology, Rhinology and Laryngology
    Volume121
    Issue number11
    DOIs
    Publication statusPublished - 2012 Nov

    Keywords

    • Denervation
    • Gene expression
    • Labyrinthectomy
    • Microarray
    • Vestibular nucleus

    ASJC Scopus subject areas

    • Otorhinolaryngology

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