Gene Silencing-mediated Resistance in Transgenic Tobacco Plants Carrying Potato Virus Y Coat Protein Gene

Sang Jae Han, Hye Sun Cho, Jin Sam You, Young Woo Nam, Eun Kyung Park, Jeong Sheop Shin, Young In Park, Won Mok Park, Kyung Hee Paek

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Unlike other pathogens, plant viruses are hardly controlled by chemical agents. Potato virus Y (PVY) is distributed around the world, and causes a great loss economically. In an attempt to minimize the damage by viruses, the PVY coat protein (CP) gene was introduced into tobacco by Agrobacterium-mediated transformation. A significant proportion of the transgenic plants displayed resistance to PVY and showed substantially decreased CP transgene expression at both protein and steady-state mRNA levels compared to susceptible transgenic or nontransgenic plants. A resistant plant was selected and self-fertilized for several generations until T 4 progenitor lines were obtained. Most of these T 4 plants accumulated extremely low levels of CP protein and steady-state mRNA, and exhibited almost complete resistance to PVY. DNA gel blot analysis revealed that the transgenic plants typically had two or three copies of the transgene. These results are characteristic of pathogen-derived resistance, in which the resistance against virus is the consequence of post-transcriptional gene silencing directed by homologous transgenes. To uncover factors that may play roles in gene silencing, sequences in the 3′ part of the transcribed region of the CP gene were transcribed in vitro and the RNA fragments were incubated with cell extracts from transgenic plants. A ribonuclease activity was detected that appeared to be specific for this transcript in the PVY-resistant transgenic plants.

Original languageEnglish
Pages (from-to)376-383
Number of pages8
JournalMolecules and Cells
Volume9
Issue number4
Publication statusPublished - 1999 Aug 31

Fingerprint

Potyvirus
Genetically Modified Plants
Gene Silencing
Tobacco
Capsid Proteins
Transgenes
Genes
Viruses
Plant Viruses
Messenger RNA
Agrobacterium
Ribonucleases
RNA Interference
Cell Extracts
Proteins
Gels
RNA
Potato virus Y coat protein
DNA

Keywords

  • Homology-dependent Gene Silencing
  • Pathogen-derived Resistance
  • Potato Virus Y
  • Tobacco (Nicotiana tabacum)
  • Transgenic Plant

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Gene Silencing-mediated Resistance in Transgenic Tobacco Plants Carrying Potato Virus Y Coat Protein Gene. / Han, Sang Jae; Cho, Hye Sun; You, Jin Sam; Nam, Young Woo; Park, Eun Kyung; Shin, Jeong Sheop; Park, Young In; Park, Won Mok; Paek, Kyung Hee.

In: Molecules and Cells, Vol. 9, No. 4, 31.08.1999, p. 376-383.

Research output: Contribution to journalArticle

Han, SJ, Cho, HS, You, JS, Nam, YW, Park, EK, Shin, JS, Park, YI, Park, WM & Paek, KH 1999, 'Gene Silencing-mediated Resistance in Transgenic Tobacco Plants Carrying Potato Virus Y Coat Protein Gene', Molecules and Cells, vol. 9, no. 4, pp. 376-383.
Han, Sang Jae ; Cho, Hye Sun ; You, Jin Sam ; Nam, Young Woo ; Park, Eun Kyung ; Shin, Jeong Sheop ; Park, Young In ; Park, Won Mok ; Paek, Kyung Hee. / Gene Silencing-mediated Resistance in Transgenic Tobacco Plants Carrying Potato Virus Y Coat Protein Gene. In: Molecules and Cells. 1999 ; Vol. 9, No. 4. pp. 376-383.
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