Generation and analysis of a complete mutant set for the Arabidopsis FT/TFL1 family shows specific effects on thermo-sensitive flowering regulation

Wanhui Kim, Tae Im Park, Seong Jeon Yoo, A. Rim Jun, Ji Hoon Ahn

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The FLOWERING LOCUS T (FT)/TERMINAL FLOWER 1 (TFL1) family proteins play an important role in the regulation of flowering time. In the Arabidopsis thaliana genome, there are six genes in the FT/TFL1 family. To determine how these FT/TFL1 family genes contribute to the regulation of flowering time, this study generated a comprehensive set of mutants (sixty-three multiple mutants in all combinations) of the FT/TFL1 family genes and analysed their flowering times at 23 and 16°C under long-day conditions. The analysis confirmed that FT and TFL1 are major determinants of flowering time under long-day conditions. At 23 °C, ft-10 tsf-1 mft-2 showed the latest flowering, whereas tfl1-20 atc-2 bft-2 showed the earliest flowering. Flowering occurred in the sextuple mutants. Introduction of tsf-1 led to reduced sensitivity to ambient temperature change. Introduction of tfl1-20 caused a stronger effect in accelerating flowering time at 16 °C than at 23 °C. Overexpression of miR156 did not block flowering of sextuple mutants, suggesting that there is a pathway to induce flowering independent of the FT/TFL1 pathway and miR156 pathway. This study proposes that this mutant population will be useful in further investigation of the functions of the FT/TFL1 family genes in plant development.

Original languageEnglish
Pages (from-to)1715-1729
Number of pages15
JournalJournal of Experimental Botany
Volume64
Issue number6
DOIs
Publication statusPublished - 2013 Apr 1

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Arabidopsis
flowering
flowers
mutants
loci
Genes
Time and Motion Studies
Plant Development
Genome
genes
Temperature
Population
Proteins
plant development
ambient temperature

Keywords

  • Arabidopsis thaliana
  • ATC
  • BFT
  • flowering time
  • FT
  • MFT
  • miR156
  • TFL1
  • TSF.

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Generation and analysis of a complete mutant set for the Arabidopsis FT/TFL1 family shows specific effects on thermo-sensitive flowering regulation. / Kim, Wanhui; Park, Tae Im; Yoo, Seong Jeon; Jun, A. Rim; Ahn, Ji Hoon.

In: Journal of Experimental Botany, Vol. 64, No. 6, 01.04.2013, p. 1715-1729.

Research output: Contribution to journalArticle

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