Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex

Byoung Kwon Park, Sony Maharjan, Su In Lee, Jinsoo Kim, Joon Yong Bae, Man-Seong Park, Hyung Joo Kwon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) uses the spike (S) glycoprotein to recognize and enter target cells. In this study, we selected two epitope peptide sequences within the receptor binding domain (RBD) of the MERS-CoV S protein. We used a complex consisting of the epitope peptide of the MERS-CoV S protein and CpG-DNA encapsulated in liposome complex to immunize mice, and produced the monoclonal antibodies 506-2G10G5 and 492-1G10E4E2. The western blotting data showed that both monoclonal antibodies detected the S protein and immunoprecipitated the native form of the S protein. Indirect immunofluorescence and confocal analysis suggested strong reactivity of the antibodies towards the S protein of MERS-CoV virus infected Vero cells. Furthermore, the 506-2G10G5 monoclonal antibody significantly reduced plaque formation in MERS-CoV infected Vero cells compared to normal mouse IgG and 492-1G10E4E2. Thus, we successfully produced a monoclonal antibody directed against the RBD domain of the S protein which could be used in the development of diagnostics and therapeutic applications in the future.

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalBMB reports
Volume52
Issue number6
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Coronavirus Spike Glycoproteins
Protein S
Liposomes
Epitopes
Monoclonal Antibodies
Peptides
DNA
Proteins
Vero Cells
Indirect Fluorescent Antibody Technique
Viruses
Middle East Respiratory Syndrome Coronavirus
Glycoproteins
Immunoglobulin G
Western Blotting
Antibodies

Keywords

  • B cell epitope
  • Lipoplex (O)
  • MERS-CoV
  • Monoclonal antibody
  • Spike protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex. / Park, Byoung Kwon; Maharjan, Sony; Lee, Su In; Kim, Jinsoo; Bae, Joon Yong; Park, Man-Seong; Kwon, Hyung Joo.

In: BMB reports, Vol. 52, No. 6, 01.01.2019, p. 397-402.

Research output: Contribution to journalArticle

Park, Byoung Kwon ; Maharjan, Sony ; Lee, Su In ; Kim, Jinsoo ; Bae, Joon Yong ; Park, Man-Seong ; Kwon, Hyung Joo. / Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex. In: BMB reports. 2019 ; Vol. 52, No. 6. pp. 397-402.
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