Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction

Jung Sik Kim, Inho Choi, Han Hyoung Lee, Suk Jun Lee, Manheum Na, Sang Hoon Kim, Jihye Han, Joonbeom Bae, Sang Pil Choi, Sang Joon Kim, Chung Gyu Park, Taehoon Chun

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4 Citations (Scopus)

Abstract

Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4; CD152) is a transmembrane protein that is structurally similar to CD28. As CTLA-4 has a much higher binding affinity to B7 than CD28, several approaches using soluble CTLA-4 have been tried to down-regulate T cell activity by blocking the interaction between CD28 and B7. We constructed soluble rhesus monkey CTLA-4 immunoglobulin (CTLA-4Ig) containing a critical binding site to B7 combined with a constant Ig heavy chain region in a mammalian system. Flow cytometry analyses indicated that soluble rhesus monkey CTLA-4Ig bound to rhesus monkey CD86 (B7. 2). Moreover, soluble rhesus monkey CTLA-4Ig more effectively blocked the rhesus monkey-rhesus monkey allogeneic mixed lymphocyte reaction compared with that of humans. These results indicate that soluble rhesus monkey CTLA-4Ig may be useful in preclinical trials in a rhesus monkey model.

Original languageEnglish
Pages (from-to)2191-2197
Number of pages7
JournalBiotechnology letters
Volume34
Issue number12
DOIs
Publication statusPublished - 2012 Nov

Keywords

  • Allogeneic response
  • Co-stimulatory molecule
  • Cytotoxic T lymphocyte-associated antigen-4
  • Mixed lymphocyte reaction
  • Rhesus monkey
  • T cells

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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    Kim, J. S., Choi, I., Lee, H. H., Lee, S. J., Na, M., Kim, S. H., Han, J., Bae, J., Choi, S. P., Kim, S. J., Park, C. G., & Chun, T. (2012). Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction. Biotechnology letters, 34(12), 2191-2197. https://doi.org/10.1007/s10529-012-1026-0