Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Myungjae Song, Jung Min Lim, Seonwoo Min, Jeong Seok Oh, Dong Young Kim, Jae Sung Woo, Hiroshi Nishimasu, Sung Rae Cho, Sungroh Yoon, Hyongbum Henry Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.

Original languageEnglish
Article number5617
JournalNature communications
Volume12
Issue number1
DOIs
Publication statusPublished - 2021 Dec 1

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • General
  • Physics and Astronomy(all)

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