Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

Hyemin Kim; Ji Woo Kim; So Jin Kim; Young Jun Choi; Dae Sung Kim; Han Jin Park

doi:10.1016/j.scr.2017.12.001

Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

Hyemin Kim, Ji Woo Kim, So Jin Kim, Young Jun Choi, Dae Sung Kim, Han Jin Park

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

Original language	English
Pages (from-to)	72-75
Number of pages	4
Journal	Stem Cell Research
Volume	26
DOIs	https://doi.org/10.1016/j.scr.2017.12.001
Publication status	Published - 2018 Jan

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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@article{299040067536435ea443b707fd44e585,

title = "Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system",

abstract = "Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.",

author = "Hyemin Kim and Kim, {Ji Woo} and Kim, {So Jin} and Choi, {Young Jun} and Kim, {Dae Sung} and Park, {Han Jin}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea (No. NRF-2015R1C1A2A01051471 ). Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea (No. NRF-2015R1C1A2A01051471).",

year = "2018",

month = jan,

doi = "10.1016/j.scr.2017.12.001",

language = "English",

volume = "26",

pages = "72--75",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

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T1 - Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

AU - Kim, Hyemin

AU - Kim, Ji Woo

AU - Kim, So Jin

AU - Choi, Young Jun

AU - Kim, Dae Sung

AU - Park, Han Jin

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea (No. NRF-2015R1C1A2A01051471 ). Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP), Republic of Korea (No. NRF-2015R1C1A2A01051471).

PY - 2018/1

Y1 - 2018/1

N2 - Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

AB - Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

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