Abstract
Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.
| Original language | English |
|---|---|
| Pages (from-to) | 72-75 |
| Number of pages | 4 |
| Journal | Stem Cell Research |
| Volume | 26 |
| DOIs | |
| Publication status | Published - 2018 Jan 1 |
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ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system. / Kim, Hyemin; Kim, Ji Woo; Kim, So Jin; Choi, Young Jun; Kim, Dae-Sung; Park, Han Jin.
In: Stem Cell Research, Vol. 26, 01.01.2018, p. 72-75.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system
AU - Kim, Hyemin
AU - Kim, Ji Woo
AU - Kim, So Jin
AU - Choi, Young Jun
AU - Kim, Dae-Sung
AU - Park, Han Jin
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.
AB - Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.
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UR - http://www.scopus.com/inward/citedby.url?scp=85037977071&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2017.12.001
DO - 10.1016/j.scr.2017.12.001
M3 - Article
C2 - 29247817
AN - SCOPUS:85037977071
VL - 26
SP - 72
EP - 75
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
ER -