Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

Hyemin Kim; Ji Woo Kim; So Jin Kim; Young Jun Choi; Dae-Sung Kim; Han Jin Park

doi:10.1016/j.scr.2017.12.001

Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

Hyemin Kim, Ji Woo Kim, So Jin Kim, Young Jun Choi, Dae-Sung Kim, Han Jin Park

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

Original language	English
Pages (from-to)	72-75
Number of pages	4
Journal	Stem Cell Research
Volume	26
DOIs	https://doi.org/10.1016/j.scr.2017.12.001
Publication status	Published - 2018 Jan 1

Fingerprint

ASJC Scopus subject areas

Developmental Biology
Cell Biology

Cite this

Kim, H., Kim, J. W., Kim, S. J., Choi, Y. J., Kim, D-S., & Park, H. J. (2018). Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system. Stem Cell Research, 26, 72-75. https://doi.org/10.1016/j.scr.2017.12.001

@article{299040067536435ea443b707fd44e585,

title = "Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system",

abstract = "Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.",

author = "Hyemin Kim and Kim, {Ji Woo} and Kim, {So Jin} and Choi, {Young Jun} and Dae-Sung Kim and Park, {Han Jin}",

year = "2018",

month = jan

day = "1",

doi = "10.1016/j.scr.2017.12.001",

language = "English",

volume = "26",

pages = "72--75",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

AU - Kim, Hyemin

AU - Kim, Ji Woo

AU - Kim, So Jin

AU - Choi, Young Jun

AU - Kim, Dae-Sung

AU - Park, Han Jin

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

AB - Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

UR - http://www.scopus.com/inward/record.url?scp=85037977071&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037977071&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2017.12.001

DO - 10.1016/j.scr.2017.12.001

M3 - Article

C2 - 29247817

AN - SCOPUS:85037977071

VL - 26

SP - 72

EP - 75

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

ER -

Access to Document

10.1016/j.scr.2017.12.001