Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

Hyemin Kim, Ji Woo Kim, So Jin Kim, Young Jun Choi, Dae-Sung Kim, Han Jin Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

Original languageEnglish
Pages (from-to)72-75
Number of pages4
JournalStem Cell Research
Volume26
DOIs
Publication statusPublished - 2018 Jan 1

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ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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