Abstract
Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.
Original language | English |
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Pages (from-to) | 72-75 |
Number of pages | 4 |
Journal | Stem Cell Research |
Volume | 26 |
DOIs | |
Publication status | Published - 2018 Jan |
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology