Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system

Hyemin Kim, Ji Woo Kim, So Jin Kim, Young Jun Choi, Dae-Sung Kim, Han Jin Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.

Original languageEnglish
Pages (from-to)72-75
Number of pages4
JournalStem Cell Research
Volume26
DOIs
Publication statusPublished - 2018 Jan 1

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Clustered Regularly Interspaced Short Palindromic Repeats
Induced Pluripotent Stem Cells
Cell Line
Hepatocytes
Pluripotent Stem Cells
Preclinical Drug Evaluations
Cytoplasmic and Nuclear Receptors
Drug-Related Side Effects and Adverse Reactions
Karyotype
Pharmaceutical Preparations
pregnane X receptor

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system. / Kim, Hyemin; Kim, Ji Woo; Kim, So Jin; Choi, Young Jun; Kim, Dae-Sung; Park, Han Jin.

In: Stem Cell Research, Vol. 26, 01.01.2018, p. 72-75.

Research output: Contribution to journalArticle

Kim, Hyemin ; Kim, Ji Woo ; Kim, So Jin ; Choi, Young Jun ; Kim, Dae-Sung ; Park, Han Jin. / Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system. In: Stem Cell Research. 2018 ; Vol. 26. pp. 72-75.
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