Abstract
Pregnane X receptor (PXR) is a key nuclear receptor that mediates drug metabolism and stimulates hepatocyte proliferation. However, the lack of PXR expression in human pluripotent stem cell-derived hepatocytes limits their application for drug screening and toxicity testing. Here, we generated a PXR-mCherry reporter human induced pluripotent stem cell (hiPSC) line using the CRISPR/Cas9 system. PXR-mCherry hiPSCs were pluripotent and had differentiation potential and a normal karyotype. This cell line is an important tool for identifying factors that increase PXR-mediated drug metabolism and hepatocyte proliferation.
| Original language | English |
|---|---|
| Pages (from-to) | 72-75 |
| Number of pages | 4 |
| Journal | Stem Cell Research |
| Volume | 26 |
| DOIs | |
| Publication status | Published - 2018 Jan 1 |
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
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Cite this
Kim, H., Kim, J. W., Kim, S. J., Choi, Y. J., Kim, D-S., & Park, H. J. (2018). Generation of a PXR reporter human induced pluripotent stem cell line (PXR-mCherry hiPSC) using the CRISPR/Cas9 system. Stem Cell Research, 26, 72-75. https://doi.org/10.1016/j.scr.2017.12.001