Generation of a WA14 hESC sub-line carrying a hemizygous ABCD1 (C.1696_1710 del) mutation introduced by CRISPR/Cas9 technology

Wonjun Hong, Wonjin Yun, Wonji Choi, Daryeon Son, Gwonhwa Song, Seungkwon You

Research output: Contribution to journalArticlepeer-review

Abstract

ATP-binding cassette transporter subfamily D member 1 (ABCD1) gene is a member of ABC transporter super family, which conduct peroxisomal import of very long chain fatty acid and crucial underlying factor that induces X-linked adrenoleukodystrophy (X-ALD) when the gene is defected. Here, we report the generation of a human embryonic stem cell sub-line harboring a hemizygous ABCD1 mutation (C.1696_1710 del) using CRISPR/Cas9 system. Established line expresses pluripotency marker genes, can be differentiated to three germ layers, and maintains a normal karyotype.

Original languageEnglish
Article number102244
JournalStem Cell Research
Volume52
DOIs
Publication statusPublished - 2021 Apr

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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