Abstract
ATP-binding cassette transporter subfamily D member 1 (ABCD1) gene is a member of ABC transporter super family, which conduct peroxisomal import of very long chain fatty acid and crucial underlying factor that induces X-linked adrenoleukodystrophy (X-ALD) when the gene is defected. Here, we report the generation of a human embryonic stem cell sub-line harboring a hemizygous ABCD1 mutation (C.1696_1710 del) using CRISPR/Cas9 system. Established line expresses pluripotency marker genes, can be differentiated to three germ layers, and maintains a normal karyotype.
Original language | English |
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Article number | 102244 |
Journal | Stem Cell Research |
Volume | 52 |
DOIs | |
Publication status | Published - 2021 Apr |
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology