Generation of an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using CRISPR/Cas9-mediated homologous recombination

Seongyea Jo, Ji Woo Kim, Haneul Noh, Hyemin Kim, Jong Hoon Kim, Han Jin Park

Research output: Contribution to journalArticlepeer-review

Abstract

Alpha-smooth muscle actin (α-SMA) is encoded by ACTA2 and is a key protein in the cellular contractile system of various mesodermal cell types, including hepatic stellate cells (HSCs), smooth muscle cells, and cardiomyocytes. α-SMA, which is a key protein in the development of hepatic fibrosis, is widely used as a reliable marker of HSC activation. Here, we generated an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using a CRISPR/Cas9-based knock-in system. These reporter hiPSC lines can be used for lineage tracing of mesodermal cells and for screening of HSC activation factors.

Original languageEnglish
Article number102524
JournalStem Cell Research
Volume56
DOIs
Publication statusPublished - 2021 Oct

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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