TY - JOUR
T1 - Generation of an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using CRISPR/Cas9-mediated homologous recombination
AU - Jo, Seongyea
AU - Kim, Ji Woo
AU - Noh, Haneul
AU - Kim, Hyemin
AU - Kim, Jong Hoon
AU - Park, Han Jin
N1 - Funding Information:
The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Han-Jin Park reports financial support was provided by National Research Foundation of Korea. Han-Jin Park reports financial support was provided by Korea Institute of Toxicology.
Funding Information:
This research was supported by the Korea Institute of Toxicology [grant number 1711133839 ] and Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT [grant number 2018M3A9H1021384 ].
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/10
Y1 - 2021/10
N2 - Alpha-smooth muscle actin (α-SMA) is encoded by ACTA2 and is a key protein in the cellular contractile system of various mesodermal cell types, including hepatic stellate cells (HSCs), smooth muscle cells, and cardiomyocytes. α-SMA, which is a key protein in the development of hepatic fibrosis, is widely used as a reliable marker of HSC activation. Here, we generated an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using a CRISPR/Cas9-based knock-in system. These reporter hiPSC lines can be used for lineage tracing of mesodermal cells and for screening of HSC activation factors.
AB - Alpha-smooth muscle actin (α-SMA) is encoded by ACTA2 and is a key protein in the cellular contractile system of various mesodermal cell types, including hepatic stellate cells (HSCs), smooth muscle cells, and cardiomyocytes. α-SMA, which is a key protein in the development of hepatic fibrosis, is widely used as a reliable marker of HSC activation. Here, we generated an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using a CRISPR/Cas9-based knock-in system. These reporter hiPSC lines can be used for lineage tracing of mesodermal cells and for screening of HSC activation factors.
UR - http://www.scopus.com/inward/record.url?scp=85113999124&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2021.102524
DO - 10.1016/j.scr.2021.102524
M3 - Article
C2 - 34481189
AN - SCOPUS:85113999124
VL - 56
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
M1 - 102524
ER -