Generation of Best1 knockdown mice using lentiviral vector expressing small hairpin RNA

Changjoon Lee, Joo Ok Min, Bo Eun Yoon

Research output: Contribution to journalArticle

Abstract

We demonstrate that pronuclear injection of lentiviral vectors expressing small hairpin RNAs (shRNAs) that silence the expression of specific genes can be used to generate knockdown mice. A lentiviral vector capable of generating shRNA that is specific for the target gene bestrophin 1 (Best1) encodes for an anion channel that is permeable to glutamate and gamma amino butyric acid (GABA) and that also regulates intracellular calcium signaling. We confirmed that cultured cerebellar glia from these Best1 knockdown mice showed attenuation of GABA release induced by an increase in intracellular calcium. Therefore, we propose that a combined approach, the use of transgenesis together with lentiviral vectors expressing shRNAs, can successfully generate a large number of mice in which the expression of a specific gene can be downregulated gradually. We also suggest that the Best1 knockdown mouse can be a useful tool for studying Best1 gene function.

Original languageEnglish
Pages (from-to)178-182
Number of pages5
JournalAnimal Cells and Systems
Volume18
Issue number3
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Animal Science and Zoology

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