Generation of cytochrome P450 polymorphic human induced pluripotent stem cell lines with defective CYP activities

Jaehun Lee, Dong Hun Woo, Han Jin Park, Duck Sung Ko, Jong-Hoon Kim

Research output: Contribution to journalArticle

Abstract

Single nucleotide polymorphisms (SNPs) in cytochrome P450 (CYP) isoenzymes alter drug metabolism and pharmacodynamics. In particular, several SNPs within CYPs decrease CYP activities, resulting in a high plasma concentration of drugs and increasing adverse effect of commonly used drugs. Here, we generated two different human induced pluripotent stem cell (hiPSC) lines, which retain defective CYP2C19 or CYP3A5 activities individually. These two hiPSC lines could be valuable sources for understading the interindividual variability in drug responses caused by SNP-induced alteration in CYP activites.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalStem Cell Research
Volume31
DOIs
Publication statusPublished - 2018 Aug 1

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Induced Pluripotent Stem Cells
Cytochrome P-450 Enzyme System
Single Nucleotide Polymorphism
Cell Line
Pharmaceutical Preparations
Cytochrome P-450 CYP3A
Isoenzymes

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Generation of cytochrome P450 polymorphic human induced pluripotent stem cell lines with defective CYP activities. / Lee, Jaehun; Woo, Dong Hun; Park, Han Jin; Ko, Duck Sung; Kim, Jong-Hoon.

In: Stem Cell Research, Vol. 31, 01.08.2018, p. 117-121.

Research output: Contribution to journalArticle

Lee, Jaehun ; Woo, Dong Hun ; Park, Han Jin ; Ko, Duck Sung ; Kim, Jong-Hoon. / Generation of cytochrome P450 polymorphic human induced pluripotent stem cell lines with defective CYP activities. In: Stem Cell Research. 2018 ; Vol. 31. pp. 117-121.
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