Generation of viable embryos and embryonic stem cell-like cells from cultured primary follicles in mice

Jun Hee Choi, Gil Ah Kim, Jong Heum Park, Gwonhwa Song, Jun Won Park, Dae Yong Kim, Jeong Mook Lim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Primary follicles retrieved from B6CBAF1 prepubertal mice were cultured in a stepwise manner in an alpha-minimum essential medium-based medium to generate viable embryos and embryonic stem cell (ESC)-like cells. A significant increase in follicle growth and oocyte maturation accompanied by increased secretion of 17beta-estradiol and progesterone was achieved by exposing primary follicles to 100 or 200 mIU of follicle-stimulating hormone (FSH) during culture. More oocytes developed into blastocysts following in vitro fertilization (IVF) or parthenogenetic activation after culture with 200 mIU of FSH during the entire culture period than with 100 mIU. Eleven ESClike cell lines, consisting of four heterozygotic and seven homozygotic phenotypes, were established from 25 trials of primary follicle culture combined with IVF or parthenogenetic activation. In conclusion, primary follicles can potentially yield developmentally competent oocytes, which produce viable embryos and ESC-like cell lines following in vitro manipulation. We suggest a method to utilize immature follicles, which are most abundant in ovaries, to improve reproductive efficiency and for use in regenerative medicine.

Original languageEnglish
Pages (from-to)744-754
Number of pages11
JournalBiology of Reproduction
Volume85
Issue number4
DOIs
Publication statusPublished - 2011 Oct 1
Externally publishedYes

Fingerprint

Embryonic Stem Cells
Oocytes
Cultured Cells
Embryonic Structures
Follicle Stimulating Hormone
Fertilization in Vitro
Cell Line
Regenerative Medicine
Blastocyst
Progesterone
Ovary
Estradiol
Phenotype
Growth

Keywords

  • Early development
  • Embryonic stem cells
  • Follicle-stimulating hormone (FSH)
  • Follicular development
  • In vitro fertilization
  • In vitro maturation (IVM)
  • MII oocytes oocyte development
  • Parthenogenesis
  • Primary follicle

ASJC Scopus subject areas

  • Cell Biology

Cite this

Generation of viable embryos and embryonic stem cell-like cells from cultured primary follicles in mice. / Choi, Jun Hee; Kim, Gil Ah; Park, Jong Heum; Song, Gwonhwa; Park, Jun Won; Kim, Dae Yong; Lim, Jeong Mook.

In: Biology of Reproduction, Vol. 85, No. 4, 01.10.2011, p. 744-754.

Research output: Contribution to journalArticle

Choi, Jun Hee ; Kim, Gil Ah ; Park, Jong Heum ; Song, Gwonhwa ; Park, Jun Won ; Kim, Dae Yong ; Lim, Jeong Mook. / Generation of viable embryos and embryonic stem cell-like cells from cultured primary follicles in mice. In: Biology of Reproduction. 2011 ; Vol. 85, No. 4. pp. 744-754.
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