Genetic alterations in intrahepatic cholangiocarcinoma as revealed by degenerate oligonucleotide primed PCR-comparative genomic hybridization

Ji Young Lee, Young Nyun Park, Kyung Ok Uhm, Soo Yeun Park, Sun Hwa Park

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Intrahepatic cholangiocarcinoma (ICC), a malignant neoplasm of the biliary epithelium, is usually fatal because of difficulty in early diagnosis and lack of availability of effective therapy. The genetic mechanisms involved in the development of ICC are not well understood and only a few cytogenetic studies of ICC have been published. Recently, technique of degenerate oligonucleotide primed (DOP)-PCR comparative genomic hybridization (CGH) permits genetic imbalances screening of the entire genome using only small amounts of tumor DNA. In this study chromosomal aberrations in 33 Korean ICC were investigated by DOP-PCR CGH. The common sites of copy number increases were 20q (67%), 17 (61%), 11q11-q13 (42%), 8p12-qter (39%), 18p (39%), 15q22-qter (36%), 16p (36%), 6p21 (30%), 3q25-qter (27%), 1q41-qter (24%), and 5p14-q11.2 (24%). DNA amplification was identified in 16 carcinomas (48%). The frequent sites of amplification were 20q, 17p, 17q23-qter, and 7p. The most frequent sites of copy number decreases were 1p32-pter (21%) and 4q (21%). The recurrent chromosomal aberrations identified in this study provide candidate regions involved in the tumorigenesis and progression of ICC.

Original languageEnglish
Pages (from-to)682-687
Number of pages6
JournalJournal of Korean medical science
Volume19
Issue number5
DOIs
Publication statusPublished - 2004 Oct

Keywords

  • Cholangiocarcinoma
  • Chromosome Aberrations
  • Comparative Genomic Hybridization
  • Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

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