Genetic analysis of human parainfluenza viruses circulating in Korea, 2006

Kwang Sook Park, Mi Hwa Yang, Chang Kyu Lee, Ki-Joon Song

Research output: Contribution to journalArticle

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Abstract

Human parainfluenza viruses (HPIV) are important causes of respiratory tract infections in young children. To characterize the molecular epidemiology of an HPIV outbreak occurring in Korea during 2006, genetic analysis of 269 cell culture isolates from HPIV-infected children, was conducted using nested reverse transcription-PCR (RT-PCR). HPIV-1 was detected in 70.3% of tested samples (189/269). The detection rate of HPIV-2 and HPIV-3 was 1.5% (4/269) and 9.3% (25/269), respectively. Mixed HPIV-1, -2 and -3 infections were detected in 19.0% (51/269): HPIV-1 and HPIV-2 in 15, HPIV-1 and HPIV-3 in 26, HPIV-2 and HPIV-3 in 6, and HPIV-1, -2 and -3 in 4. Of these positive samples for three different types HIPV-1, -2, and -3, two each representative strains were selected, the full length of hemagglutinin-neuraminidase (HN) gene for HPIV was amplified by RT-PCR, and sequenced. Multiple alignment analysis, based on reference sequence of HPIV-1, -2, and -3 strains available in GenBank, showed that the identity of nucleotide and deduced amino acid sequences was 92.4-97.6% and 92.7-97.9%, respectively, for HPIV-1, 88.5-99.8% and 88.6-100% for HPIV-2, and 96.3-99.5% and 95.0-99.3% for HPIV-3, respectively. Phylogenetic analysis showed that HPIV-1, -2, and -3 strains identified in this study were closely related among the strains in the same type with no significant genetic variability. These results show that HPIV of multiple imported sources was circulating in Korea.

Original languageEnglish
Pages (from-to)1041-1047
Number of pages7
JournalJournal of Medical Virology
Volume86
Issue number6
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Human parainfluenza virus 2
Human parainfluenza virus 1
Paramyxoviridae Infections
Medical Genetics
Korea
Human parainfluenza virus 3
Viruses
Reverse Transcription
Polymerase Chain Reaction
Molecular Epidemiology
Nucleic Acid Databases
Hemagglutinins
Neuraminidase
Respiratory Tract Infections
Disease Outbreaks
Amino Acid Sequence
Nucleotides
Cell Culture Techniques

Keywords

  • Hemagglutinin-neuraminidase gene
  • Mixed infection
  • Parainfluenza virus
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Genetic analysis of human parainfluenza viruses circulating in Korea, 2006. / Park, Kwang Sook; Yang, Mi Hwa; Lee, Chang Kyu; Song, Ki-Joon.

In: Journal of Medical Virology, Vol. 86, No. 6, 01.01.2014, p. 1041-1047.

Research output: Contribution to journalArticle

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abstract = "Human parainfluenza viruses (HPIV) are important causes of respiratory tract infections in young children. To characterize the molecular epidemiology of an HPIV outbreak occurring in Korea during 2006, genetic analysis of 269 cell culture isolates from HPIV-infected children, was conducted using nested reverse transcription-PCR (RT-PCR). HPIV-1 was detected in 70.3{\%} of tested samples (189/269). The detection rate of HPIV-2 and HPIV-3 was 1.5{\%} (4/269) and 9.3{\%} (25/269), respectively. Mixed HPIV-1, -2 and -3 infections were detected in 19.0{\%} (51/269): HPIV-1 and HPIV-2 in 15, HPIV-1 and HPIV-3 in 26, HPIV-2 and HPIV-3 in 6, and HPIV-1, -2 and -3 in 4. Of these positive samples for three different types HIPV-1, -2, and -3, two each representative strains were selected, the full length of hemagglutinin-neuraminidase (HN) gene for HPIV was amplified by RT-PCR, and sequenced. Multiple alignment analysis, based on reference sequence of HPIV-1, -2, and -3 strains available in GenBank, showed that the identity of nucleotide and deduced amino acid sequences was 92.4-97.6{\%} and 92.7-97.9{\%}, respectively, for HPIV-1, 88.5-99.8{\%} and 88.6-100{\%} for HPIV-2, and 96.3-99.5{\%} and 95.0-99.3{\%} for HPIV-3, respectively. Phylogenetic analysis showed that HPIV-1, -2, and -3 strains identified in this study were closely related among the strains in the same type with no significant genetic variability. These results show that HPIV of multiple imported sources was circulating in Korea.",
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