Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport

G. T. Hayman, S. B. Von Bodman, Heenam Kim, P. Jiang, S. K. Farrand

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The acc region, subcloned from pTiC58 of classical nopaline and agrocinopine A and B Agrobacterium tumefaciens C58, allowed agrobacteria to grow using agrocinopine B as the sole source of carbon and energy. acc is approximately 6 kb in size. It consists of at least five genes, accA through accE, as defined by complementation analysis using subcloned fragments and transposon insertion mutations of acc carried on different plasmids within the same cell. All five regions are required for agrocin 84 sensitivity, and at least four are required for agrocinopine and agrocin 84 uptake. The complementation results are consistent with the hypothesis that each of the five regions is separately transcribed. Maxicell experiments showed that the first of these genes, accA, encodes a 60-kDa protein. Analysis of osmotic shock fractions showed this protein to be located in the periplasm. The DNA sequence of the accA region revealed an open reading frame encoding a predicted polypeptide of 59,147 Da. The amino acid sequence encoded by this open reading frame is similar to the periplasmic binding proteins OppA and DppA of Escherichia coli and Salmonella typhimurium and OppA of Bacillus subtilis.

Original languageEnglish
Pages (from-to)5575-5584
Number of pages10
JournalJournal of Bacteriology
Volume175
Issue number17
Publication statusPublished - 1993 Sep 17
Externally publishedYes

Fingerprint

Plant Tumor-Inducing Plasmids
Open Reading Frames
Periplasmic Binding Proteins
Periplasm
Agrobacterium tumefaciens
Agrobacterium
Insertional Mutagenesis
Osmotic Pressure
Salmonella typhimurium
Bacillus subtilis
Genes
Amino Acid Sequence
Proteins
Plasmids
Carbon
Escherichia coli
Peptides
agrocin 84
agrocinopine
agrocinopin B

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport. / Hayman, G. T.; Von Bodman, S. B.; Kim, Heenam; Jiang, P.; Farrand, S. K.

In: Journal of Bacteriology, Vol. 175, No. 17, 17.09.1993, p. 5575-5584.

Research output: Contribution to journalArticle

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abstract = "The acc region, subcloned from pTiC58 of classical nopaline and agrocinopine A and B Agrobacterium tumefaciens C58, allowed agrobacteria to grow using agrocinopine B as the sole source of carbon and energy. acc is approximately 6 kb in size. It consists of at least five genes, accA through accE, as defined by complementation analysis using subcloned fragments and transposon insertion mutations of acc carried on different plasmids within the same cell. All five regions are required for agrocin 84 sensitivity, and at least four are required for agrocinopine and agrocin 84 uptake. The complementation results are consistent with the hypothesis that each of the five regions is separately transcribed. Maxicell experiments showed that the first of these genes, accA, encodes a 60-kDa protein. Analysis of osmotic shock fractions showed this protein to be located in the periplasm. The DNA sequence of the accA region revealed an open reading frame encoding a predicted polypeptide of 59,147 Da. The amino acid sequence encoded by this open reading frame is similar to the periplasmic binding proteins OppA and DppA of Escherichia coli and Salmonella typhimurium and OppA of Bacillus subtilis.",
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AU - Farrand, S. K.

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