Genetic and molecular epidemiological characterization of a novel adenovirus in antarctic penguins collected between 2008 and 2013

Sook Young Lee, Jeong Hoon Kim, Tae Kun Seo, Jin Sun No, Han Kyeom Kim, Wonkeun Kim, Han Gu Choi, Sung Ho Kang, Jin Won Song

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Antarctica is considered a relatively uncontaminated region with regard to the infectious diseases because of its extreme environment, and isolated geography. For the genetic characterization and molecular epidemiology of the newly found penguin adenovirus in Antarctica, entire genome sequencing and annual survey of penguin adenovirus were conducted. The entire genome sequences of penguin adenoviruses were completed for two Chinstrap penguins (Pygoscelis antarctica) and two Gentoo penguins (Pygoscelis papua). The whole genome lengths and G+C content of penguin adenoviruses were found to be 24,630-24,662 bp and 35.5-35.6%, respectively. Notably, the presence of putative sialidase gene was not identified in penguin adenoviruses by Rapid Amplification of cDNA Ends (RACE-PCR) as well as consensus specific PCR. The penguin adenoviruses were demonstrated to be a new species within the genus Siadenovirus, with a distance of 29.9-39.3% (amino acid, 32.1-47.9%) in DNA polymerase gene, and showed the closest relationship with Turkey adenovirus 3 (TAdV-3) in phylogenetic analysis. During the 2008-2013 study period, the penguin adenoviruses were annually detected in 22 of 78 penguins (28.2%), and the molecular epidemiological study of the penguin adenovirus indicates a predominant infection in Chinstrap penguin population (12/30, 40%). Interestingly, the genome of penguin adenovirus could be detected in several internal samples, except the lymph node and brain. In conclusion, an analysis of the entire adenoviral genomes from Antarctic penguins was conducted, and the penguin adenoviruses, containing unique genetic character, were identified as a new species within the genus Siadenovirus. Moreover, it was annually detected in Antarctic penguins, suggesting its circulation within the penguin population.

Original languageEnglish
Article numbere0157032
JournalPLoS One
Volume11
Issue number6
DOIs
Publication statusPublished - 2016 Jun 1

Fingerprint

Spheniscidae
penguins
Adenoviridae
Molecular Biology
Genes
Siadenovirus
Genome
genome
Antarctica
Neuraminidase
Pygoscelis
DNA-Directed DNA Polymerase
Molecular Epidemiology
Amplification
Turkey adenovirus A
Brain
Complementary DNA
Amino Acids
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Genetic and molecular epidemiological characterization of a novel adenovirus in antarctic penguins collected between 2008 and 2013. / Lee, Sook Young; Kim, Jeong Hoon; Seo, Tae Kun; No, Jin Sun; Kim, Han Kyeom; Kim, Wonkeun; Choi, Han Gu; Kang, Sung Ho; Song, Jin Won.

In: PLoS One, Vol. 11, No. 6, e0157032, 01.06.2016.

Research output: Contribution to journalArticle

Lee, Sook Young ; Kim, Jeong Hoon ; Seo, Tae Kun ; No, Jin Sun ; Kim, Han Kyeom ; Kim, Wonkeun ; Choi, Han Gu ; Kang, Sung Ho ; Song, Jin Won. / Genetic and molecular epidemiological characterization of a novel adenovirus in antarctic penguins collected between 2008 and 2013. In: PLoS One. 2016 ; Vol. 11, No. 6.
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abstract = "Antarctica is considered a relatively uncontaminated region with regard to the infectious diseases because of its extreme environment, and isolated geography. For the genetic characterization and molecular epidemiology of the newly found penguin adenovirus in Antarctica, entire genome sequencing and annual survey of penguin adenovirus were conducted. The entire genome sequences of penguin adenoviruses were completed for two Chinstrap penguins (Pygoscelis antarctica) and two Gentoo penguins (Pygoscelis papua). The whole genome lengths and G+C content of penguin adenoviruses were found to be 24,630-24,662 bp and 35.5-35.6{\%}, respectively. Notably, the presence of putative sialidase gene was not identified in penguin adenoviruses by Rapid Amplification of cDNA Ends (RACE-PCR) as well as consensus specific PCR. The penguin adenoviruses were demonstrated to be a new species within the genus Siadenovirus, with a distance of 29.9-39.3{\%} (amino acid, 32.1-47.9{\%}) in DNA polymerase gene, and showed the closest relationship with Turkey adenovirus 3 (TAdV-3) in phylogenetic analysis. During the 2008-2013 study period, the penguin adenoviruses were annually detected in 22 of 78 penguins (28.2{\%}), and the molecular epidemiological study of the penguin adenovirus indicates a predominant infection in Chinstrap penguin population (12/30, 40{\%}). Interestingly, the genome of penguin adenovirus could be detected in several internal samples, except the lymph node and brain. In conclusion, an analysis of the entire adenoviral genomes from Antarctic penguins was conducted, and the penguin adenoviruses, containing unique genetic character, were identified as a new species within the genus Siadenovirus. Moreover, it was annually detected in Antarctic penguins, suggesting its circulation within the penguin population.",
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