Genetic characterization of avian metapneumovirus subtype C isolated from pheasants in a live bird market

Eun ho Lee, Min Suk Song, Jin Young Shin, Young Min Lee, Chul Joong Kim, Youngsik Lee, Hyunggee Kim, Young Ki Choi

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Abstract

Complete nucleotide sequences of two avian metapneumoviruses (aMPV), designated PL-1 and PL-2, were isolated from pheasants, revealing novel sequences of the first aMPV to be fully sequenced in Korea. The complete genome of both PL-1 and PL-2 was composed of 13,170 nucleotides. Phylogenetic analysis revealed that PL-1 belonged to aMPV subtype C, sharing higher homology in deduced amino acid sequence identities with hMPV, rather than with aMPV subtypes A and B. Replication of PL-1 in experimentally re-infected pheasants was confirmed by reverse transcription (RT)-polymerase chain reaction (PCR). Chickens and mice were experimentally inoculated with PL-1 to test the replication potential of PL-1 in other species. Although one specimen from the nasal turbinates of an inoculated chicken showed a slight trace of viral replication at 3 days post-infection (dpi), all of the infected mice were negative for aMPV by RT-PCR throughout the experiment, suggesting that PL-1 does not readily infect mammals. This is the first report of the isolation and complete genomic sequence of aMPV subtype C originating from pheasants.

Original languageEnglish
Pages (from-to)18-25
Number of pages8
JournalVirus Research
Volume128
Issue number1-2
DOIs
Publication statusPublished - 2007 Sep 1

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Keywords

  • Avian metapneumovirus
  • Gene sequence
  • Korea
  • Pheasant

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

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