Genetic distribution of group A human rotavirus types isolated in Gyunggi province of Korea, 1999-2002

Sung Sil Moon, Yangsook Song Green, Jin Won Song, Chang Nam Ahn, Hoon Kim, Kwang Sook Park, Ki-Joon Song, Jung Hwa Lee, Luck Ju Baek

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Human rotavirus genotypes G1-G4 and G9 are the major etiological agents of infantile gastroenteritis. G1 was the most prevalent in Korea during the 10-year period prior to 1997. However, between 1998 and 1999, G4 was the predominant type in Korea, as it was in other Asian countries. Objectives: The circulating pattern and genetic variability of group A human rotavirus in Gyunggi, Korea, 1999-2002, were examined in 189 stool specimens. Study design: Stool samples were collected from children with diarrhea, and group A human rotavirus type was determined using multiplex RT-PCR in those specimens found to be positive for rotavirus by ELISA. Each genotype was sequenced, and phylogenetic analysis was performed on the sequences. Result: We found significant variability from year to year in the prevalence of different G and P types of rotavirus. We also found relatively high prevalence rates for types normally considered to be uncommon. Furthermore, we found that the most prevalent combination of G and P types changed from year to year. Although the combination of G and P types changed every year, the sequence of G genotypes showed a high level of similarity (>97%) compared to those of strains from other Asian countries. Conclusion: We report the types of rotavirus circulating in Gyunggi province, Korea from 1999 to 2002. This information on rotavirus diversity has important implications for rotavirus vaccine efficacy and future vaccine development.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalJournal of Clinical Virology
Volume38
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Rotavirus
Korea
Genotype
Rotavirus Vaccines
Multiplex Polymerase Chain Reaction
Gastroenteritis
Diarrhea
Vaccines
Enzyme-Linked Immunosorbent Assay

Keywords

  • Genotype
  • Group A human rotavirus
  • Phylogenetic analysis
  • VP4 (P)
  • VP7 (G)

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Genetic distribution of group A human rotavirus types isolated in Gyunggi province of Korea, 1999-2002. / Moon, Sung Sil; Green, Yangsook Song; Song, Jin Won; Ahn, Chang Nam; Kim, Hoon; Park, Kwang Sook; Song, Ki-Joon; Lee, Jung Hwa; Baek, Luck Ju.

In: Journal of Clinical Virology, Vol. 38, No. 1, 01.01.2007, p. 57-63.

Research output: Contribution to journalArticle

Moon, Sung Sil ; Green, Yangsook Song ; Song, Jin Won ; Ahn, Chang Nam ; Kim, Hoon ; Park, Kwang Sook ; Song, Ki-Joon ; Lee, Jung Hwa ; Baek, Luck Ju. / Genetic distribution of group A human rotavirus types isolated in Gyunggi province of Korea, 1999-2002. In: Journal of Clinical Virology. 2007 ; Vol. 38, No. 1. pp. 57-63.
@article{fb98311db9384f72b92657880805849c,
title = "Genetic distribution of group A human rotavirus types isolated in Gyunggi province of Korea, 1999-2002",
abstract = "Background: Human rotavirus genotypes G1-G4 and G9 are the major etiological agents of infantile gastroenteritis. G1 was the most prevalent in Korea during the 10-year period prior to 1997. However, between 1998 and 1999, G4 was the predominant type in Korea, as it was in other Asian countries. Objectives: The circulating pattern and genetic variability of group A human rotavirus in Gyunggi, Korea, 1999-2002, were examined in 189 stool specimens. Study design: Stool samples were collected from children with diarrhea, and group A human rotavirus type was determined using multiplex RT-PCR in those specimens found to be positive for rotavirus by ELISA. Each genotype was sequenced, and phylogenetic analysis was performed on the sequences. Result: We found significant variability from year to year in the prevalence of different G and P types of rotavirus. We also found relatively high prevalence rates for types normally considered to be uncommon. Furthermore, we found that the most prevalent combination of G and P types changed from year to year. Although the combination of G and P types changed every year, the sequence of G genotypes showed a high level of similarity (>97{\%}) compared to those of strains from other Asian countries. Conclusion: We report the types of rotavirus circulating in Gyunggi province, Korea from 1999 to 2002. This information on rotavirus diversity has important implications for rotavirus vaccine efficacy and future vaccine development.",
keywords = "Genotype, Group A human rotavirus, Phylogenetic analysis, VP4 (P), VP7 (G)",
author = "Moon, {Sung Sil} and Green, {Yangsook Song} and Song, {Jin Won} and Ahn, {Chang Nam} and Hoon Kim and Park, {Kwang Sook} and Ki-Joon Song and Lee, {Jung Hwa} and Baek, {Luck Ju}",
year = "2007",
month = "1",
day = "1",
doi = "10.1016/j.jcv.2006.10.004",
language = "English",
volume = "38",
pages = "57--63",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Genetic distribution of group A human rotavirus types isolated in Gyunggi province of Korea, 1999-2002

AU - Moon, Sung Sil

AU - Green, Yangsook Song

AU - Song, Jin Won

AU - Ahn, Chang Nam

AU - Kim, Hoon

AU - Park, Kwang Sook

AU - Song, Ki-Joon

AU - Lee, Jung Hwa

AU - Baek, Luck Ju

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Background: Human rotavirus genotypes G1-G4 and G9 are the major etiological agents of infantile gastroenteritis. G1 was the most prevalent in Korea during the 10-year period prior to 1997. However, between 1998 and 1999, G4 was the predominant type in Korea, as it was in other Asian countries. Objectives: The circulating pattern and genetic variability of group A human rotavirus in Gyunggi, Korea, 1999-2002, were examined in 189 stool specimens. Study design: Stool samples were collected from children with diarrhea, and group A human rotavirus type was determined using multiplex RT-PCR in those specimens found to be positive for rotavirus by ELISA. Each genotype was sequenced, and phylogenetic analysis was performed on the sequences. Result: We found significant variability from year to year in the prevalence of different G and P types of rotavirus. We also found relatively high prevalence rates for types normally considered to be uncommon. Furthermore, we found that the most prevalent combination of G and P types changed from year to year. Although the combination of G and P types changed every year, the sequence of G genotypes showed a high level of similarity (>97%) compared to those of strains from other Asian countries. Conclusion: We report the types of rotavirus circulating in Gyunggi province, Korea from 1999 to 2002. This information on rotavirus diversity has important implications for rotavirus vaccine efficacy and future vaccine development.

AB - Background: Human rotavirus genotypes G1-G4 and G9 are the major etiological agents of infantile gastroenteritis. G1 was the most prevalent in Korea during the 10-year period prior to 1997. However, between 1998 and 1999, G4 was the predominant type in Korea, as it was in other Asian countries. Objectives: The circulating pattern and genetic variability of group A human rotavirus in Gyunggi, Korea, 1999-2002, were examined in 189 stool specimens. Study design: Stool samples were collected from children with diarrhea, and group A human rotavirus type was determined using multiplex RT-PCR in those specimens found to be positive for rotavirus by ELISA. Each genotype was sequenced, and phylogenetic analysis was performed on the sequences. Result: We found significant variability from year to year in the prevalence of different G and P types of rotavirus. We also found relatively high prevalence rates for types normally considered to be uncommon. Furthermore, we found that the most prevalent combination of G and P types changed from year to year. Although the combination of G and P types changed every year, the sequence of G genotypes showed a high level of similarity (>97%) compared to those of strains from other Asian countries. Conclusion: We report the types of rotavirus circulating in Gyunggi province, Korea from 1999 to 2002. This information on rotavirus diversity has important implications for rotavirus vaccine efficacy and future vaccine development.

KW - Genotype

KW - Group A human rotavirus

KW - Phylogenetic analysis

KW - VP4 (P)

KW - VP7 (G)

UR - http://www.scopus.com/inward/record.url?scp=33845455746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845455746&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2006.10.004

DO - 10.1016/j.jcv.2006.10.004

M3 - Article

C2 - 17118702

AN - SCOPUS:33845455746

VL - 38

SP - 57

EP - 63

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 1

ER -