Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis

Hanna Lee, Seong Jeon Yoo, Jeong Hwan Lee, Wanhui Kim, Seung Kwan Yoo, Heather Fitzgerald, James C. Carrington, Ji Hoon Ahn

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Abstract

Flowering is the primary trait affected by ambient temperature changes. Plant microRNAs (miRNAs) are small non-coding RNAs playing an important regulatory role in plant development. In this study, to elucidate the mechanism of flowering-time regulation by small RNAs, we identified six ambient temperature-responsive miRNAs (miR156, miR163, miR169, miR172, miR398 and miR399) in Arabidopsis via miRNA microarray and northern hybridization analyses. We also determined the expression profile of 120 unique miRNA loci in response to ambient temperature changes by miRNA northern hybridization analysis. The expression of the ambient temperature-responsive miRNAs and their target genes was largely anticorrelated at two different temperatures (16 and 23oC). Interestingly, a lesion in short vegetative phase (SVP), a key regulator within the thermosensory pathway, caused alteration in the expression of miR172 and a subset of its target genes, providing a link between a thermosensory pathway gene and miR172. The miR172-overexpressing plants showed a temperatureindependent early flowering phenotype, suggesting that modulation of miR172 expression leads to temperature insensitivity. Taken together, our results suggest a genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs under non-stress temperature conditions.

Original languageEnglish
Article numbergkp1240
Pages (from-to)3081-3093
Number of pages13
JournalNucleic acids research
Volume38
Issue number9
DOIs
Publication statusPublished - 2010 Jan 27

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ASJC Scopus subject areas

  • Genetics

Cite this

Lee, H., Yoo, S. J., Lee, J. H., Kim, W., Yoo, S. K., Fitzgerald, H., Carrington, J. C., & Ahn, J. H. (2010). Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis. Nucleic acids research, 38(9), 3081-3093. [gkp1240]. https://doi.org/10.1093/nar/gkp1240