Genetic interactions reveal the antagonistic roles of FT/TSF and TFL1 in the determination of inflorescence meristem identity in Arabidopsis

Chunghee Lee, Soo Jin Kim, Suhyun Jin, Hendry Susila, Geummin Youn, Zeeshan Nasim, Hemasundar Alavilli, Kyung Sook Chung, Seong Jeon Yoo, Ji Hoon Ahn

Research output: Contribution to journalArticle

Abstract

During the transition to the reproductive phase, the shoot apical meristem switches from the developmental program that generates vegetative organs to instead produce flowers. In this study, we examined the genetic interactions of FLOWERING LOCUS T (FT)/TWIN SISTER OF FT (TSF) and TERMINAL FLOWER 1 (TFL1) in the determination of inflorescence meristem identity in Arabidopsis thaliana. The ft-10 tsf-1 mutants produced a compact inflorescence surrounded by serrated leaves (hyper-vegetative shoot) at the early bolting stage, as did plants overexpressing TFL1. Plants overexpressing FT or TSF (or both FT and TFL1) generated a terminal flower, as did tfl1-20 mutants. The terminal flower formed in tfl1-20 mutants converted to a hyper-vegetative shoot in ft-10 tsf-1 mutants. Grafting ft-10 tsf-1 or ft-10 tsf-1 tfl1-20 mutant scions to 35S::FT rootstock plants produced a normal inflorescence and a terminal flower in the scion plants, respectively, although both scions showed similar early flowering. Misexpression of FT in the vasculature and in the shoot apex in wild-type plants generated a normal inflorescence and a terminal flower, respectively. By contrast, in ft-10 tsf-1 mutants the vasculature-specific misexpression of FT converted the hyper-vegetative shoot to a normal inflorescence, and in the ft-10 tsf-1 tfl1-20 mutants converted the shoot to a terminal flower. TFL1 levels did not affect the inflorescence morphology caused by FT/TSF overexpression at the early bolting stage. Taking these results together, we proposed that FT/TSF and TFL1 play antagonistic roles in the determination of inflorescence meristem identity, and that FT/TSF are more important than TFL1 in this process.

Original languageEnglish
JournalPlant Journal
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Inflorescence
Meristem
Arabidopsis
meristems
inflorescences
flowering
loci
mutants
flowers
scions
shoots
bolting
shoot meristems
grafting (plants)
apical meristems
rootstocks
Angiospermae
Arabidopsis thaliana

Keywords

  • floral homeotic transformation
  • FT
  • hyper-vegetative shoot
  • inflorescence meristem identity
  • misexpression
  • TFL1
  • TSF

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Genetic interactions reveal the antagonistic roles of FT/TSF and TFL1 in the determination of inflorescence meristem identity in Arabidopsis. / Lee, Chunghee; Kim, Soo Jin; Jin, Suhyun; Susila, Hendry; Youn, Geummin; Nasim, Zeeshan; Alavilli, Hemasundar; Chung, Kyung Sook; Yoo, Seong Jeon; Ahn, Ji Hoon.

In: Plant Journal, 01.01.2019.

Research output: Contribution to journalArticle

Lee, Chunghee ; Kim, Soo Jin ; Jin, Suhyun ; Susila, Hendry ; Youn, Geummin ; Nasim, Zeeshan ; Alavilli, Hemasundar ; Chung, Kyung Sook ; Yoo, Seong Jeon ; Ahn, Ji Hoon. / Genetic interactions reveal the antagonistic roles of FT/TSF and TFL1 in the determination of inflorescence meristem identity in Arabidopsis. In: Plant Journal. 2019.
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AU - Youn, Geummin

AU - Nasim, Zeeshan

AU - Alavilli, Hemasundar

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