Genetic relationships of cattle breeds assessed by PCR-RFLP of the bovine mitochondrial DNA D-loop region

Hak Yoon Du, Kyo Lee Hak, Jung O. Sung, Ki Chang Hong, Gwang Joo Jeon, Sik Kong Hong, Heon Lee Jun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asian Bos indicus, and 2 African Bos indicus. The size of amplified PCR products from mtDNA D-loop region was 964 bp and the products were digested by 15 different restriction enzymes. Two different band patterns were identified in eight restriction enzymes (BstXI, Hae III, Msp I, Apa I, Tag I, Alu I, BamH I, EcoN I) and the rest of restriction enzymes showed more than 3 different band patterns among which Apo I and MspR9 resulted in 7 different restriction patterns. The genotypes, number of haplotype, effective number of haplotype, and degree of heterozygosity were analyzed. Based on all the PCR-RFLP data, different haplotypes were constructed and analyzed for calculating genetic distances between these breeds using Nei's unbiased method and constructing a phylogenetic tree.

Original languageEnglish
Pages (from-to)1368-1374
Number of pages7
JournalAsian-Australasian Journal of Animal Sciences
Volume18
Issue number10
Publication statusPublished - 2005 Oct 1

Fingerprint

cattle breeds
Mitochondrial DNA
Restriction Fragment Length Polymorphisms
genetic relationships
haplotypes
restriction fragment length polymorphism
mitochondrial DNA
zebu
Polymerase Chain Reaction
cattle
enzymes
Haplotypes
genetic distance
heterozygosity
Enzymes
breeds
genotype
phylogeny
animals
Genotype

Keywords

  • Bos Indicus
  • Bos Taurus
  • Genetic Distance
  • Mitochondrial DNA
  • PCR-RFLP

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Genetic relationships of cattle breeds assessed by PCR-RFLP of the bovine mitochondrial DNA D-loop region. / Du, Hak Yoon; Hak, Kyo Lee; Sung, Jung O.; Hong, Ki Chang; Jeon, Gwang Joo; Hong, Sik Kong; Jun, Heon Lee.

In: Asian-Australasian Journal of Animal Sciences, Vol. 18, No. 10, 01.10.2005, p. 1368-1374.

Research output: Contribution to journalArticle

Du, Hak Yoon ; Hak, Kyo Lee ; Sung, Jung O. ; Hong, Ki Chang ; Jeon, Gwang Joo ; Hong, Sik Kong ; Jun, Heon Lee. / Genetic relationships of cattle breeds assessed by PCR-RFLP of the bovine mitochondrial DNA D-loop region. In: Asian-Australasian Journal of Animal Sciences. 2005 ; Vol. 18, No. 10. pp. 1368-1374.
@article{ea35e45224b44b7da7aa9ecb9710218a,
title = "Genetic relationships of cattle breeds assessed by PCR-RFLP of the bovine mitochondrial DNA D-loop region",
abstract = "To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asian Bos indicus, and 2 African Bos indicus. The size of amplified PCR products from mtDNA D-loop region was 964 bp and the products were digested by 15 different restriction enzymes. Two different band patterns were identified in eight restriction enzymes (BstXI, Hae III, Msp I, Apa I, Tag I, Alu I, BamH I, EcoN I) and the rest of restriction enzymes showed more than 3 different band patterns among which Apo I and MspR9 resulted in 7 different restriction patterns. The genotypes, number of haplotype, effective number of haplotype, and degree of heterozygosity were analyzed. Based on all the PCR-RFLP data, different haplotypes were constructed and analyzed for calculating genetic distances between these breeds using Nei's unbiased method and constructing a phylogenetic tree.",
keywords = "Bos Indicus, Bos Taurus, Genetic Distance, Mitochondrial DNA, PCR-RFLP",
author = "Du, {Hak Yoon} and Hak, {Kyo Lee} and Sung, {Jung O.} and Hong, {Ki Chang} and Jeon, {Gwang Joo} and Hong, {Sik Kong} and Jun, {Heon Lee}",
year = "2005",
month = "10",
day = "1",
language = "English",
volume = "18",
pages = "1368--1374",
journal = "Asian-Australasian Journal of Animal Sciences",
issn = "1011-2367",
publisher = "Asian-Australasian Association of Animal Production Societies",
number = "10",

}

TY - JOUR

T1 - Genetic relationships of cattle breeds assessed by PCR-RFLP of the bovine mitochondrial DNA D-loop region

AU - Du, Hak Yoon

AU - Hak, Kyo Lee

AU - Sung, Jung O.

AU - Hong, Ki Chang

AU - Jeon, Gwang Joo

AU - Hong, Sik Kong

AU - Jun, Heon Lee

PY - 2005/10/1

Y1 - 2005/10/1

N2 - To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asian Bos indicus, and 2 African Bos indicus. The size of amplified PCR products from mtDNA D-loop region was 964 bp and the products were digested by 15 different restriction enzymes. Two different band patterns were identified in eight restriction enzymes (BstXI, Hae III, Msp I, Apa I, Tag I, Alu I, BamH I, EcoN I) and the rest of restriction enzymes showed more than 3 different band patterns among which Apo I and MspR9 resulted in 7 different restriction patterns. The genotypes, number of haplotype, effective number of haplotype, and degree of heterozygosity were analyzed. Based on all the PCR-RFLP data, different haplotypes were constructed and analyzed for calculating genetic distances between these breeds using Nei's unbiased method and constructing a phylogenetic tree.

AB - To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asian Bos indicus, and 2 African Bos indicus. The size of amplified PCR products from mtDNA D-loop region was 964 bp and the products were digested by 15 different restriction enzymes. Two different band patterns were identified in eight restriction enzymes (BstXI, Hae III, Msp I, Apa I, Tag I, Alu I, BamH I, EcoN I) and the rest of restriction enzymes showed more than 3 different band patterns among which Apo I and MspR9 resulted in 7 different restriction patterns. The genotypes, number of haplotype, effective number of haplotype, and degree of heterozygosity were analyzed. Based on all the PCR-RFLP data, different haplotypes were constructed and analyzed for calculating genetic distances between these breeds using Nei's unbiased method and constructing a phylogenetic tree.

KW - Bos Indicus

KW - Bos Taurus

KW - Genetic Distance

KW - Mitochondrial DNA

KW - PCR-RFLP

UR - http://www.scopus.com/inward/record.url?scp=26844574384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26844574384&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 1368

EP - 1374

JO - Asian-Australasian Journal of Animal Sciences

JF - Asian-Australasian Journal of Animal Sciences

SN - 1011-2367

IS - 10

ER -