Genetic structures of invasive Streptococcus pneumoniae isolates from Korean children obtained between 1995 and 2013

Ki Wook Yun, Eun Hwa Choi, Hoan Jong Lee, Jin Han Kang, Kyung Hyo Kim, Dong Soo Kim, Yae Jean Kim, Byung Wook Eun, Sung Hee Oh, Hye Kyung Cho, Young Jin Hong, Kwang Nam Kim, Nam Hee Kim, Yun Kyung Kim, Hyunju Lee, Taekjin Lee, Hwang Min Kim, Eun Young Cho, Chun Soo Kim, Su Eun ParkChi Eun Oh, Dae Sun Jo, Young Youn Choi, Jina Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Understanding the population genetics of pneumococci will allow detection of changes in the prevalence of circulating genotypes and evidence for capsular switching. We aimed to analyze the genetic structure of invasive pneumococcal isolates obtained from children before and after the use of pneumococcal conjugate vaccines (PCVs) in Korea. Methods: A total of 285 invasive pneumococcal isolates were analyzed using serotyping, multilocus sequence typing, and antimicrobial susceptibility testing. We classified the isolation year to pre-PCV7 (1995-2003; n = 70), post-PCV7 (2004-2010; n = 142), and post-PCV13 (2011-2013; n = 73) periods. Results: Of the 10 clonal complexes (CCs), antibiotic-resistant international clones, CC320 (31.6%), CC81 (14.7%), and CC166 (6.7%) were the main complexes. Serotype 19A was the main serotype of CC320 throughout the periods. Serotypes of CC81 mainly comprised of 23F (53.3%) in pre-PCV7 period and replaced by non-vaccine types (NVTs; 6C [10%], 13 [30%], 15A [40%], and 15B/C [20%]) in post-PCV13 period. The main serotype responsible for CC166 also changed from 9 V (80%) in pre-PCV7 to NVT 11A (50%) in post-PCV13 periods. Non-susceptibility to penicillin (42.3%) was the highest in CC320, increasing from 0 to 76%. Conclusion: The genetic structures of invasive pneumococcal isolates in Korean children have changed concomitantly with serotype after the implementation of PCVs.

Original languageEnglish
Article number268
JournalBMC Infectious Diseases
Volume18
Issue number1
DOIs
Publication statusPublished - 2018 Jun 8

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Genetic Structures
Streptococcus pneumoniae
Conjugate Vaccines
Pneumococcal Vaccines
Multilocus Sequence Typing
Serotyping
Population Genetics
Korea
Penicillins
Clone Cells
Genotype
Serogroup
Anti-Bacterial Agents

Keywords

  • Children
  • Invasive pneumococcal diseases
  • Multilocus sequence typing
  • Pneumococcal conjugate vaccine

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Genetic structures of invasive Streptococcus pneumoniae isolates from Korean children obtained between 1995 and 2013. / Yun, Ki Wook; Choi, Eun Hwa; Lee, Hoan Jong; Kang, Jin Han; Kim, Kyung Hyo; Kim, Dong Soo; Kim, Yae Jean; Eun, Byung Wook; Oh, Sung Hee; Cho, Hye Kyung; Hong, Young Jin; Kim, Kwang Nam; Kim, Nam Hee; Kim, Yun Kyung; Lee, Hyunju; Lee, Taekjin; Kim, Hwang Min; Cho, Eun Young; Kim, Chun Soo; Park, Su Eun; Oh, Chi Eun; Jo, Dae Sun; Choi, Young Youn; Lee, Jina.

In: BMC Infectious Diseases, Vol. 18, No. 1, 268, 08.06.2018.

Research output: Contribution to journalArticle

Yun, KW, Choi, EH, Lee, HJ, Kang, JH, Kim, KH, Kim, DS, Kim, YJ, Eun, BW, Oh, SH, Cho, HK, Hong, YJ, Kim, KN, Kim, NH, Kim, YK, Lee, H, Lee, T, Kim, HM, Cho, EY, Kim, CS, Park, SE, Oh, CE, Jo, DS, Choi, YY & Lee, J 2018, 'Genetic structures of invasive Streptococcus pneumoniae isolates from Korean children obtained between 1995 and 2013', BMC Infectious Diseases, vol. 18, no. 1, 268. https://doi.org/10.1186/s12879-018-3177-7
Yun, Ki Wook ; Choi, Eun Hwa ; Lee, Hoan Jong ; Kang, Jin Han ; Kim, Kyung Hyo ; Kim, Dong Soo ; Kim, Yae Jean ; Eun, Byung Wook ; Oh, Sung Hee ; Cho, Hye Kyung ; Hong, Young Jin ; Kim, Kwang Nam ; Kim, Nam Hee ; Kim, Yun Kyung ; Lee, Hyunju ; Lee, Taekjin ; Kim, Hwang Min ; Cho, Eun Young ; Kim, Chun Soo ; Park, Su Eun ; Oh, Chi Eun ; Jo, Dae Sun ; Choi, Young Youn ; Lee, Jina. / Genetic structures of invasive Streptococcus pneumoniae isolates from Korean children obtained between 1995 and 2013. In: BMC Infectious Diseases. 2018 ; Vol. 18, No. 1.
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abstract = "Background: Understanding the population genetics of pneumococci will allow detection of changes in the prevalence of circulating genotypes and evidence for capsular switching. We aimed to analyze the genetic structure of invasive pneumococcal isolates obtained from children before and after the use of pneumococcal conjugate vaccines (PCVs) in Korea. Methods: A total of 285 invasive pneumococcal isolates were analyzed using serotyping, multilocus sequence typing, and antimicrobial susceptibility testing. We classified the isolation year to pre-PCV7 (1995-2003; n = 70), post-PCV7 (2004-2010; n = 142), and post-PCV13 (2011-2013; n = 73) periods. Results: Of the 10 clonal complexes (CCs), antibiotic-resistant international clones, CC320 (31.6{\%}), CC81 (14.7{\%}), and CC166 (6.7{\%}) were the main complexes. Serotype 19A was the main serotype of CC320 throughout the periods. Serotypes of CC81 mainly comprised of 23F (53.3{\%}) in pre-PCV7 period and replaced by non-vaccine types (NVTs; 6C [10{\%}], 13 [30{\%}], 15A [40{\%}], and 15B/C [20{\%}]) in post-PCV13 period. The main serotype responsible for CC166 also changed from 9 V (80{\%}) in pre-PCV7 to NVT 11A (50{\%}) in post-PCV13 periods. Non-susceptibility to penicillin (42.3{\%}) was the highest in CC320, increasing from 0 to 76{\%}. Conclusion: The genetic structures of invasive pneumococcal isolates in Korean children have changed concomitantly with serotype after the implementation of PCVs.",
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T1 - Genetic structures of invasive Streptococcus pneumoniae isolates from Korean children obtained between 1995 and 2013

AU - Yun, Ki Wook

AU - Choi, Eun Hwa

AU - Lee, Hoan Jong

AU - Kang, Jin Han

AU - Kim, Kyung Hyo

AU - Kim, Dong Soo

AU - Kim, Yae Jean

AU - Eun, Byung Wook

AU - Oh, Sung Hee

AU - Cho, Hye Kyung

AU - Hong, Young Jin

AU - Kim, Kwang Nam

AU - Kim, Nam Hee

AU - Kim, Yun Kyung

AU - Lee, Hyunju

AU - Lee, Taekjin

AU - Kim, Hwang Min

AU - Cho, Eun Young

AU - Kim, Chun Soo

AU - Park, Su Eun

AU - Oh, Chi Eun

AU - Jo, Dae Sun

AU - Choi, Young Youn

AU - Lee, Jina

PY - 2018/6/8

Y1 - 2018/6/8

N2 - Background: Understanding the population genetics of pneumococci will allow detection of changes in the prevalence of circulating genotypes and evidence for capsular switching. We aimed to analyze the genetic structure of invasive pneumococcal isolates obtained from children before and after the use of pneumococcal conjugate vaccines (PCVs) in Korea. Methods: A total of 285 invasive pneumococcal isolates were analyzed using serotyping, multilocus sequence typing, and antimicrobial susceptibility testing. We classified the isolation year to pre-PCV7 (1995-2003; n = 70), post-PCV7 (2004-2010; n = 142), and post-PCV13 (2011-2013; n = 73) periods. Results: Of the 10 clonal complexes (CCs), antibiotic-resistant international clones, CC320 (31.6%), CC81 (14.7%), and CC166 (6.7%) were the main complexes. Serotype 19A was the main serotype of CC320 throughout the periods. Serotypes of CC81 mainly comprised of 23F (53.3%) in pre-PCV7 period and replaced by non-vaccine types (NVTs; 6C [10%], 13 [30%], 15A [40%], and 15B/C [20%]) in post-PCV13 period. The main serotype responsible for CC166 also changed from 9 V (80%) in pre-PCV7 to NVT 11A (50%) in post-PCV13 periods. Non-susceptibility to penicillin (42.3%) was the highest in CC320, increasing from 0 to 76%. Conclusion: The genetic structures of invasive pneumococcal isolates in Korean children have changed concomitantly with serotype after the implementation of PCVs.

AB - Background: Understanding the population genetics of pneumococci will allow detection of changes in the prevalence of circulating genotypes and evidence for capsular switching. We aimed to analyze the genetic structure of invasive pneumococcal isolates obtained from children before and after the use of pneumococcal conjugate vaccines (PCVs) in Korea. Methods: A total of 285 invasive pneumococcal isolates were analyzed using serotyping, multilocus sequence typing, and antimicrobial susceptibility testing. We classified the isolation year to pre-PCV7 (1995-2003; n = 70), post-PCV7 (2004-2010; n = 142), and post-PCV13 (2011-2013; n = 73) periods. Results: Of the 10 clonal complexes (CCs), antibiotic-resistant international clones, CC320 (31.6%), CC81 (14.7%), and CC166 (6.7%) were the main complexes. Serotype 19A was the main serotype of CC320 throughout the periods. Serotypes of CC81 mainly comprised of 23F (53.3%) in pre-PCV7 period and replaced by non-vaccine types (NVTs; 6C [10%], 13 [30%], 15A [40%], and 15B/C [20%]) in post-PCV13 period. The main serotype responsible for CC166 also changed from 9 V (80%) in pre-PCV7 to NVT 11A (50%) in post-PCV13 periods. Non-susceptibility to penicillin (42.3%) was the highest in CC320, increasing from 0 to 76%. Conclusion: The genetic structures of invasive pneumococcal isolates in Korean children have changed concomitantly with serotype after the implementation of PCVs.

KW - Children

KW - Invasive pneumococcal diseases

KW - Multilocus sequence typing

KW - Pneumococcal conjugate vaccine

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