Genetic variability in Plasmodium vivax aldolase gene in Korean isolates and the sensitivity of the Binax Now malaria test

Chy Hyun Cho, Myung-Hyun Nam, Jang Su Kim, Eun Taek Han, Won Ja Lee, Jun Seo Oh, Seong Soo A An, Chae Seung Lim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Introduction of rapid malaria diagnostic tests (RDT) initiated numerous field evaluations in various epidemiologic settings. But the efficiency of some RTD kits based on aldolase raised reservations for direct implementation of RDT into clinical settings. We performed Binax Now malaria test in 84 Korean Plasmodium vivax isolates and compared it with the traditional Giemsa stain microscopy test as the reference standard. The sensitivity of Binax Now was 62.0% for P. vivax cases (52/84, 95% CI 51.2-71.6%) with 100.0% specificity (50/50, 95% confidence interval 92.9-100%). After the aldolase gene sequence analysis of 84 isolates, two synonymous mutations in aldolase gene were identified in both Binax Now positive and negative samples. No significant association between the mutations and Binax Now malaria tests was found. Thus, the genetic variability would not explain the poor performance of P. vivax RDTs by detecting aldolase in ROK isolates.

Original languageEnglish
Pages (from-to)223-226
Number of pages4
JournalTropical Medicine and International Health
Volume16
Issue number2
DOIs
Publication statusPublished - 2011 Feb 1

Fingerprint

Plasmodium vivax
Fructose-Bisphosphate Aldolase
Malaria
Routine Diagnostic Tests
Genes
Azure Stains
Sequence Analysis
Microscopy
Confidence Intervals
Mutation

Keywords

  • Aldolase
  • Binax Now
  • OptiMAL
  • Plasmodium vivax
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases
  • Parasitology

Cite this

Genetic variability in Plasmodium vivax aldolase gene in Korean isolates and the sensitivity of the Binax Now malaria test. / Cho, Chy Hyun; Nam, Myung-Hyun; Kim, Jang Su; Han, Eun Taek; Lee, Won Ja; Oh, Jun Seo; An, Seong Soo A; Lim, Chae Seung.

In: Tropical Medicine and International Health, Vol. 16, No. 2, 01.02.2011, p. 223-226.

Research output: Contribution to journalArticle

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