One hundred forty-six fecal specimens collected between 2007 and 2008 from infants with acute gastroenteritis were screened for rotavirus by ELISA with VP6-specific antibody. One hundred twenty-three of the samples (84.2%) were confirmed to be positive for group A rotavirus (community-acquired, n=90 [73.2%] and nosocomial, n=33 [26.8%]), and were typed subsequently using RT-PCR and sequence analysis methods. Determination of G- and P-type combinations showed that G4P (78.9%) was the most common strain, followed by G3P (7.3%), G1P (6.5%), G2P (0.8%), G2P (0.8%), G1P (0.8%), and G9P (0.8%) strains. Of the 97 G4P strains, 62 (63.8%) were responsible for community-acquired cases and 35 (36.1%) were hospital-acquired cases. Phylogenetic analysis of the VP7 gene from the G4P strains revealed that both the community-acquired and nosocomial strains were segregated to thehuman rotaviruses circulating worldwide, including the prototype vaccinal strain, ST3, which constituted a novel sublineage in lineage 1. Owing to the recent emergence of G4P rotaviruses within the hospital, as well as in the community, the findings from this study are important since they provide new information concerning the community and nosocomial spread of rotaviruses.
ASJC Scopus subject areas
- Infectious Diseases